User guide

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CHAPTER 2 Prepare Samples and Load Reactions .................. 36
Prepare the DNA samples ....................................................... 36
Quality of DNA ............................................................. 36
Quantity of DNA ........................................................... 36
Sample dilution ............................................................ 37
Determine the optimal dilution when the target is known ....................... 37
Determine the optimal dilution when the target is unknown ..................... 38
Prepare the digital PCR reactions ................................................ 38
Required materials ........................................................ 38
Guidelines for PCR sample preparation ....................................... 38
Prepare the reaction mix and samples ....................................... 39
Load the Digital PCR 20K Chips .................................................. 40
Guidelines for loading and sealing Digital PCR 20K Chips ....................... 41
Load the chips using the Chip Loader ............................................ 42
Materials required ......................................................... 42
QuantStudio
®
3D Digital PCR Chip Loader status light .......................... 42
Load and seal the chips using a Chip Loader .................................. 43
Load chips manually ............................................................ 50
Materials required ......................................................... 50
Load and seal the Digital PCR 20K Chips ...................................... 51
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CHAPTER 3 Perform the PCR ......................................... 58
Choosing a thermal cycler ...................................................... 58
Compatible thermal cyclers ................................................. 58
Prepare the thermal cycler ................................................. 59
Thermal cycle the Digital PCR 20K Chips .......................................... 61
Materials required ......................................................... 61
Guidelines for handling Chip Cases .......................................... 62
Perform the PCR .......................................................... 62
Unload the thermal cycler .................................................. 64
Contents
4 QuantStudio
®
3D Digital PCR System User Guide