User guide

If you... Then...
do not want to protect your methods press Accept (F1) again.
want to protect your methods see the GeneAmp
®
PCR System 9700
Base Module User Manual
(Pub. no. 4303481) for instructions on
securing your methods.
want to return to the Main menu
without adding the new user name
press Cancel (F5).
9.
Program the GeneAmp
®
PCR System 9700 with the method for thermal cycling
QuantStudio
®
3D Digital PCR 20K Chip
s:
Table 2 GeneAmp
®
PCR System 9700 PCR Method
PCR Protocol
Run
Speed
Reaction
Volume
Stage 1 Stage 2 Stage 3
96.0°C 60.0°C 98.0°C 60.0°C 10.0°C
[1]
Standard 20 μL10:00 2:00 0:30 2:00 99:59
1x (Hold) 39x (Cycles) 1x (Hold)
[1]
O
ptional step.
2 Holds2 Tmp 39 Cycles1 Hold
96.0
10:00
60.0
2:00
60.0
2:00
98.0
0:30
10.0
Start
Return
Method: exp 001
F1 F3 F4 F5F2
Note: For information on programming the GeneAmp
®
PCR System 9700, see
the GeneAmp
®
PCR System 9700
Base Module User Manual (Pub. no. 4303481).
The installation of the GeneAmp
®
PCR System 9700 is complete and the components
are ready for use.
Connect the QuantStudio
®
3D Instrument to a network
If you choose not to connect the QuantStudio
®
3D Instrument using the setup wizard
during installation, you can connect it to a network at any time afterwards to
streamline data transfer to the QuantStudio
®
3D AnalysisSuite
™
Software. If you
choose to connect your QuantStudio
®
3D Instrument, see Appendix B, “Networking“
for the complete installation procedure, including guidelines for integrating the
instrument into your laboratory network.
IMPORTANT! This document does not provide adequate detail to integrate the
QuantStudio
®
3D Instrument into all possible network architectures. Because your
laboratory network can contain advanced features (such as firewalls), we recommend
that you consult a network administrator before connecting your instrument.
Chapter 1 Getting Started
Connect the QuantStudio
®
3D Instrument to a network
1
34 QuantStudio
®
3D Digital PCR System User Guide