User guide

Table Of Contents
Contents
About this guide ............................................................ 8
Revision history ................................................................. 8
Purpose ....................................................................... 8
Prerequisites ................................................................... 9
Instrument hardware ............................................................ 9
Instrument software ............................................................. 9
System requirements ...................................................... 10
Instrument consumables ....................................................... 10
About the Digital PCR 20K Chip .............................................. 10
CHAPTER 1 Getting Started ............................................ 11
About the QuantStudio
®
3D Digital PCR System .................................... 11
Data collection ............................................................ 12
Instrument filters and supported dyes ........................................ 12
Operating the instrument ....................................................... 13
Compatible reagent kits and assays .......................................... 13
Operational workflow ...................................................... 13
Prepare the QuantStudio
®
3D Digital PCR System for use ........................... 14
About the installation ...................................................... 14
Materials required ......................................................... 14
Before you begin .......................................................... 14
Plan the laboratory layout .................................................. 15
Choose additional protective devices ......................................... 16
Install the QuantStudio
®
3D Digital PCR Instrument ............................ 17
Install the thermal cycler ................................................... 23
Connect the QuantStudio
®
3D Instrument to a network .............................. 34
Connect the QuantStudio
®
3D AnalysisSuite
Software ............................. 35
QuantStudio
®
3D Digital PCR System User Guide
3