User guide
Table Of Contents
- QuantStudio 3D Digital PCR System User Guide (Pub. no. MAN0007720 Rev. C.0)
- Copyright Page
- Contents
- About this guide
- Chapter 1. Getting Started
- About the QuantStudio® 3D Digital PCR System
- Operating the instrument
- Prepare the QuantStudio® 3D Digital PCR System for use
- Connect the QuantStudio® 3D Instrument to a network
- Connect the QuantStudio® 3D AnalysisSuite™ Software
- Chapter 2. Prepare Samples and Load Reactions
- Chapter 3. Perform the PCR
- Chapter 4. Analyze the Prepared Chips
- Chapter 5. Troubleshooting
- Appendix A. Maintenance
- Appendix B. Networking
- Appendix C. Parts and Materials
- Appendix D. Specifications and Layout
- Appendix E. Safety
- Documentation and support
- Back Cover
Contents
About this guide ............................................................ 8
Revision history ................................................................. 8
Purpose ....................................................................... 8
Prerequisites ................................................................... 9
Instrument hardware ............................................................ 9
Instrument software ............................................................. 9
System requirements ...................................................... 10
Instrument consumables ....................................................... 10
About the Digital PCR 20K Chip .............................................. 10
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CHAPTER 1 Getting Started ............................................ 11
About the QuantStudio
®
3D Digital PCR System .................................... 11
Data collection ............................................................ 12
Instrument filters and supported dyes ........................................ 12
Operating the instrument ....................................................... 13
Compatible reagent kits and assays .......................................... 13
Operational workflow ...................................................... 13
Prepare the QuantStudio
®
3D Digital PCR System for use ........................... 14
About the installation ...................................................... 14
Materials required ......................................................... 14
Before you begin .......................................................... 14
Plan the laboratory layout .................................................. 15
Choose additional protective devices ......................................... 16
Install the QuantStudio
®
3D Digital PCR Instrument ............................ 17
Install the thermal cycler ................................................... 23
Connect the QuantStudio
®
3D Instrument to a network .............................. 34
Connect the QuantStudio
®
3D AnalysisSuite
™
Software ............................. 35
QuantStudio
®
3D Digital PCR System User Guide
3