User guide

11.
For optimal results, create a new method from the existing 3D template supplied
with the ProFlex
™
System and verify the pre-defined method for thermal cycling
the QuantStudio
®
3D Digital PCR 20K Chips:
Table 1 ProFlex
™
2x Flat PCR System PCR Method
PCR Protocol
Cover
Temp.
Reaction
Volume
Stage 1 Stage 2 Stage 3
96.0°C 60.0°C 98.0°C 60.0°C 10.0°C
[1]
70.0°C 1 nL0:10:00 0:02:00 0:00:30 0:02:00 ∞
1x (Hold) 39x (Cycles) 1x (Hold)
[1]
O
ptional step.
Note: For information on opening templates and programming the thermal
cycler, see the ProFlex
™
PCR System User Guide (Pub. no. MAN0007697). If you
cannot locate the 3D template on your ProFlex
™
System, you may need to
upgrade the firmware (see “Prepare the thermal cycler“ on page 59).
The installation of the ProFlex
™
System is complete and the thermal cycler is ready for
use.
Install the GeneAmp
®
PCR System 9700
Note: This section includes the essential procedures required to prepare the
GeneAmp
®
PCR System 9700 for use. For complete instructions, see the GeneAmp
®
PCR System 9700 Base Module User Manual (Pub. no. 4303481) or the GeneAmp
®
PCR
System 9700 Dual Flat Block User Manual (Pub. no. 4307808).
1.
Unpack and place the GeneAmp
®
PCR System 9700 Base Module:
a.
Open the package containing the GeneAmp
®
PCR System 9700 Base
Module.
b.
Remove the packing crate from the GeneAmp
®
PCR System 9700 Package,
and verify that it contains all of the parts listed on the shipping manifest.
If you have not received one or more of the parts, contact customer support
for replacement (see “Customer and technical support“ on page 121).
Chapter 1 Getting Started
Prepare the QuantStudio
®
3D Digital PCR System for use
1
QuantStudio
®
3D Digital PCR System User Guide 29