User guide
Table Of Contents
- QuantStudio 3D Digital PCR System User Guide (Pub. no. MAN0007720 Rev. C.0)
- Copyright Page
- Contents
- About this guide
- Chapter 1. Getting Started
- About the QuantStudio® 3D Digital PCR System
- Operating the instrument
- Prepare the QuantStudio® 3D Digital PCR System for use
- Connect the QuantStudio® 3D Instrument to a network
- Connect the QuantStudio® 3D AnalysisSuite™ Software
- Chapter 2. Prepare Samples and Load Reactions
- Chapter 3. Perform the PCR
- Chapter 4. Analyze the Prepared Chips
- Chapter 5. Troubleshooting
- Appendix A. Maintenance
- Appendix B. Networking
- Appendix C. Parts and Materials
- Appendix D. Specifications and Layout
- Appendix E. Safety
- Documentation and support
- Back Cover
1.
Plan the organization of your laboratory. Identify the locations of all stations in
the QuantStudio
®
3D Digital PCR Instrument workflow, including:
• Reagent and consumable storage
• PCR reaction preparation
• QuantStudio
®
3D Digital PCR 20K Chip loading and sealing
• ProFlex
™
2x Flat PCR System (or Dual Flat Block GeneAmp
®
PCR System
9700) for PCR amplification
• QuantStudio
®
3D Digital PCR Instrument for imaging and analysis
Note: See “Plan the laboratory layout“ on page 15 for more information on
planning the instrument placement.
2.
Confirm that you have received the following materials required for the
installation of the QuantStudio
®
3D Instrument:
• QuantStudio
®
3D Digital PCR Instrument (with power cord)
• QuantStudio
®
3D Digital PCR 20K Chip Adapters
• QuantStudio
®
3D Digital PCR Thermal Pads
• QuantStudio
®
3D Digital PCR 20K Chips (12-pack)
• ProFlex
™
PCR System Base Module (or GeneAmp
®
PCR System 9700 Base
Module)
• ProFlex
™
2x Flat Sample Block (or GeneAmp
®
PCR System 9700 Dual Flat
Block Module)
• QuantStudio
®
3D Tilt Base (appropriate for your thermal cycler)
• QuantStudio
®
3D Digital PCR Chip Loader
If you have not received one or more of the items listed above, contact customer
support for a replacement (see “Customer and technical support“ on page 121).
3.
If you plan to connect your instrument to a network, confirm that you have
ordered and received either the:
• (Wired network) Category-6 Ethernet cable
• (Wireless network) SparkLAN WUBR-170GN 802.11n Single-Band Wireless
Dongle
IMPORTANT! Network setup is not required for instrument installation. If
necessary, you can connect your instrument to a network at a later time after the
installation.
4.
Confirm that all chemistry kits and accessories have been stored at the correct
temperature.
5.
Unpack and install the QuantStudio
®
3D Instrument:
a.
Open the QuantStudio
®
3D Digital PCR Instrument Package.
b.
Confirm that you have received all parts listed on the shipping manifest.
If you have not received one or more parts, contact Support for a
replacement.
Install the
QuantStudio
®
3D
Digital PCR
Instrument
Chapter 1 Getting Started
Prepare the QuantStudio
®
3D Digital PCR System for use
1
QuantStudio
®
3D Digital PCR System User Guide
17