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QuantStudio 3D Digital PCR System User Guide (Pub. no. MAN0007720 Rev. C.0)

When planning the placement of the QuantStudio

3D Digital PCR System

components, give special consideration to the design and organization of the

laboratory. To minimize the potential for environmental contamination while

preparing your dPCR reactions, we recommend organizing your laboratory so that

the area where amplified DNA is handled is physically isolated from the work areas

for DNA extraction and PCR setup. These pre- and post-PCR designated work areas,

each ideally with dedicated equipment and supplies, will allow you to maintain strict

physical isolation between the area designated for handling amplified DNA and the

area where nucleic acids are extracted and dPCR reactions are assembled, thereby

avoiding transfer of amplified DNA out of the designated work area.

Room Activities Equipment

PCR Setup Work

Area (pre-PCR

room)

• Sample preparation and DNA

extraction

• Extracted DNA sample storage

• PCR reaction preparation and

DNA sample additions

• QuantStudio

3D Digital PCR 20K

Chip loading and sealing

• General laboratory supplies (such as gloves and

wipes)

• Microcentrifuge

• Vortexer

• Pipettes, P10 to P1000 with tips

• QuantStudio

3D Digital PCR 20K Chip

• QuantStudio

3D Digital PCR Chip Case Lid

• QuantStudio

3D Digital PCR Chip Loader

• QuantStudio

3D Digital PCR Sample Loading Blade

• Immersion Fluid

• UV-Activated Chip Sealant Syringes and Tips

Amplified DNA

Work Area (post-

PCR room)

• PCR amplification

• QuantStudio

3D Digital PCR 20K

Chip reading and storage

• Sealed QuantStudio

3D Digital

PCR 20K Chip storage

• ProFlex

™

2x Flat PCR System (or Dual Flat Block

GeneAmp

PCR System 9700)

• QuantStudio

3D Digital PCR Instrument

Note: Ideally, amplified DNA or equipment and supplies used to handle amplified

DNA should not be taken out of the designated work area. If the work area for

amplified DNA is in a separate but contiguous room, make sure that air flows toward

the amplified DNA area. In addition, it is helpful if there is a separate exit from the

post-PCR work area that does not exit into the pre-PCR work area.

Note: If possible, the station for loading Digital PCR 20K Chips should be physically

separated from the PCR area, away from the ProFlex

™

System (or GeneAmp

PCR

System 9700) and the QuantStudio

3D Instrument.

Plan the

laboratory layout

Chapter 1 Getting Started

Prepare the QuantStudio

3D Digital PCR System for use

QuantStudio

3D Digital PCR System User Guide