User guide

Operating the instrument
The QuantStudio
®
3D Digital PCR System can be used to perform experiments using
compatible Applied Biosystems
®
reagent kits and assays. General information on
preparing digital PCR reactions is included in this document. For specific information
on a reagent kit or assay, refer to the documentation accompanying the product or
consult our website (see “How to order from the website“ on page 106).
The following figure shows the workflow for performing a single experiment on the
QuantStudio
®
3D Digital PCR System. The procedures for sample and/or consumable
preparation and result analysis can vary depending on the specific experiment that
you are performing.
Start
q
Set up the dPCR reaction by mixing sample, master mix, and
assay(s).
q
Load the dPCR reaction onto a QuantStudio
®
3D Digital PCR 20K
Chip, apply a lid, load the assembly with immersion fluid, then
seal the loading port.
q
Perform the PCR using the ProFlex
™
2x Flat PCR System (or Dual
Flat Block GeneAmp
®
PCR System 9700).
q
Read the Digital PCR 20K Chip using the QuantStudio
®
3D Digital
PCR Instrument.
q
Review the results on the QuantStudio
®
3D Instrument
touchscreen.
q
Store or discard the Digital PCR 20K Chip.
q
Analyze the data using the QuantStudio
®
3D AnalysisSuite
™
Software.
q
Finish
Note: Refer to the user documentation for the QuantStudio
®
3D AnalysisSuite
™
Software for detailed information on data analysis.
Compatible
reagent kits and
assays
Operational
workflow
Chapter 1 Getting Started
Operating the instrument
1
QuantStudio
®
3D Digital PCR System User Guide 13