User guide

Getting Started
■
About the QuantStudio
®
3D Digital PCR System ......................... 11
■
Operating the instrument .............................................. 13
■
Prepare the QuantStudio
®
3D Digital PCR System for use ................. 14
■
Connect the QuantStudio
®
3D Instrument to a network ................... 34
■
Connect the QuantStudio
®
3D AnalysisSuite
™
Software .................. 35
About the QuantStudio
®
3D Digital PCR System
The QuantStudio
®
3D Digital PCR System provides quantitative and qualitative
research detection of target nucleic acid sequences (targets) using post-PCR
(endpoint) analysis. The QuantStudio
®
3D Instrument performs imaging and
preliminary analysis of QuantStudio
®
3D Digital PCR 20K Chips that have been
loaded with fluorescent-labeled quantitative PCR reagents (TaqMan
®
probe-based
assays or SYBR
®
Green primer-based assays) and thermal cycled using a ProFlex
™
2x
Flat PCR System (or Dual Flat Block GeneAmp
®
PCR System 9700). Subsequent
analysis and post-processing is performed by the QuantStudio
®
3D AnalysisSuite
™
Software to yield relative or absolute quantification results from the raw imaging
data.
The following figure lists the components of the QuantStudio
®
3D System.
1
4
3
6
7
8
5
9
10 11
2
13
12
1
QuantStudio
®
3D Digital PCR Thermal
Pads
2
QuantStudio
®
3D Digital PCR Chip
Adapters
3
ProFlex
™
2x Flat PCR System (or Dual Flat
Block GeneAmp
®
PCR System 9700)
4
QuantStudio
®
3D Tilt Base
5
QuantStudio
®
3D Digital PCR Instrument
6
QuantStudio
®
3D Digital PCR Chip Case
Lid
7
QuantStudio
®
3D Digital PCR Sample
Loading Blade
8
QuantStudio
®
3D Digital PCR 20K Chip
9
(Optional) QuantStudio
®
3D Digital PCR
Chip Loader
10
UV-Activated Chip Sealant Syringe
11
Immersion Fluid
12
QuantStudio
®
3D Digital PCR Master Mix
13
Fluorescent-labeled quantitative PCR
reagents (TaqMan
®
Assays or SYBR
®
Green primers)
14
(Not shown) QuantStudio
®
3D
AnalysisSuite
™
Software
1
QuantStudio
®
3D Digital PCR System User Guide 11