User guide
Table Of Contents
- QuantStudio 3D Digital PCR System User Guide (Pub. no. MAN0007720 Rev. C.0)
- Copyright Page
- Contents
- About this guide
- Chapter 1. Getting Started
- About the QuantStudio® 3D Digital PCR System
- Operating the instrument
- Prepare the QuantStudio® 3D Digital PCR System for use
- Connect the QuantStudio® 3D Instrument to a network
- Connect the QuantStudio® 3D AnalysisSuite™ Software
- Chapter 2. Prepare Samples and Load Reactions
- Chapter 3. Perform the PCR
- Chapter 4. Analyze the Prepared Chips
- Chapter 5. Troubleshooting
- Appendix A. Maintenance
- Appendix B. Networking
- Appendix C. Parts and Materials
- Appendix D. Specifications and Layout
- Appendix E. Safety
- Documentation and support
- Back Cover
The QuantStudio
®
3D AnalysisSuite
™
Software is a web-based application that is
verified for use with the following operating systems and internet browsers.
Operating system version Browser version
Microsoft
®
Windows
®
7
32-bit, Service Pack 1 Google
®
Chrome
™
v37
64-bit, Service Pack 1 Microsoft
®
Internet Explorer
®
v10 and v11
Mozilla
®
Firefox
®
v32
Apple
®
Macintosh
®
OS X
®
v10.9.4 Apple
®
Safari
®
v6
Note: The software performance may vary based on your system configuration, and
requires an internet connection capable of 300kbps/300kbps (upload/download) or
better. If your network employs a firewall that restricts outbound traffic, it must be
configured to allow outbound access to
apps.lifetechnologies.com on HTTPS-443.
Instrument consumables
The QuantStudio
®
3D Digital PCR 20K Chip is a 10-mm
2
high-density reaction plate
that consists of a single array of 20,000 reaction wells. Hydrophobic coatings on the
chip surface enable loading and isolation of independent PCR reactions within the
reaction wells.
Once loaded with a PCR reaction, the Digital PCR 20K Chip must be sealed using a
QuantStudio
®
3D Digital PCR Chip Case Lid and filled with Immersion Fluid. The
following illustration shows the components of the Digital PCR 20K Chip assembly.
X0300036
1
3
2
5
4
6
1
Chip Case Lid – The lid used to seal the
Digital PCR 20K Chip for thermal cycling
and imaging on the QuantStudio
®
3D
Instrument.
2
Digital PCR 20K Chip – The 10-mm
2
consumable that contains the
20,000 reaction wells, which suspend the
individual PCR reactions for thermal
cycling and imaging.
3
QuantStudio
®
3D Digital PCR Chip Case –
The thermal-conductive base that secures
and protects the Digital PCR 20K Chip
during all phases of use.
4
Chip ID – A label applied to the
QuantStudio
®
3D Digital PCR Chip Case
Lid that can be used to uniquely identify
the chip to which it is applied.
5
Fill port – The aperture within the Chip
Case Lid through which Immersion Fluid
is injected onto the chip.
6
Reaction wells – The 20,000 physical
holes within the Digital PCR 20K Chip that
suspend the individual PCR reactions.
System
requirements
About the Digital
PCR 20K Chip
About this guide
Instrument consumables
10
QuantStudio
®
3D Digital PCR System User Guide