User manual

Rolera Thunder User Manual 21
Application Settings
Summary
The table below summarizes typical Rolera Thunder settings for two types of applications and is provided as
a setup aid. Actual settings will vary based on the exact nature of your experiment design.
Application
Readout
Amplifier
(Port)
Readout
Speed
Multiplication
Gain
Conversion
Gain
(e-/ADU) Readout Mode
Kinetic samples
(single molecule
fluorescence or
tracking)
Multiplication
(Port #1)
10 MHz or
5 MHz
Use as required to
obtain best
signal-to-noise
ratio (SNR).
Typical EM gain
values are around
200.
#2: 6e
-
/ADU Overlap mode
(Frame Transfer,
Pre-Sequence)
Fixed samples
(bright field)
Non-
multiplication
(Port #2)
1.25 MHz Not Applicable #1: 4e
-
/ADU Overlap or Non-
overlap
Table 2. Application Type and Rolera Thunder Setup Parameters