User guide

Running a Protocol
User Guide | 9 7
Running a Protocol
To run a protocol
 Click Run Protocol in the left pane.
The software runs through the steps in imaging mode, imaging each channel. A
message at the bottom of the scr
een indicates the channel being imaged and a
progress indicator tracks the process.
Note: If you run a chemiluminescence application using autoexposure, you
might see the following err
or message:
The sample is too faint to use the autoexposure setting. Change the Image
Exposur
e setting to use manual exposure or Signal Accumulation Mode (SAM), then
run the protocol again.
This error occurs if Image Lab, using the initial autoexposure algorithm, determines
that it would take too long (appr
oximately 30 minutes or more) to acquire an image.
You can always use the manual Image Exposure setting and specify a longer
exposure time.
After the images are taken, Image Lab continues with the detection, analysis, and
r
eporting steps if these steps were selected in the Protocol Setup pane. When
Image Lab has completed running the protocol, it displays the images that were
acquired. You can then edit and save these images or do further analysis on them.
To end the protocol
 Click Cancel Run.