User guide

Creating a Protocol
User Guide | 7 9
Choose Custom to save and run an existing application with a new name or to
create and run a new application. Previously saved custom applications display
here. To create a custom application, see Setting Up a Custom Application on
page 100.
Tip: For a list of applications with all required detection reagents, light
sour
ces, and any conversion screens or filters noted, see Application
Tables on page 103.
Note: If you select the Stain Free option, you may select the gel activation
time. See Appendix E, Using the Criterion Stain Free System, on page 253
for more information.
2. In Imaging Area, select from the list of Bio-Rad gels or enter the
image area
dimensions. The red box represents the imaging area for the selected gel, and
the gray rectangle represents the imager sample stage.
3. In Image Exposure, select from one of the following options:
 Auto Exposure — this setting estimates an optimal exposure time and
ensures the best use of the dynamic range. Intense Bands optimizes for all
bands and Faint Bands makes faint bands more visible but might
overexpose more prominent bands.
 Intense Bands — optimizes exposure for all bands
 Faint Bands — a longer exposure time is used making faint bands
more visible, but more prominent bands might be overexposed