User manual

5
© 2010 Nexcelom Bioscience LLC
Table of Contents
Part I
Introduction and Description
8
................................................................................................................................... 91 Cellometer counting chamber
................................................................................................................................... 9
2 Cell counting steps
................................................................................................................................... 9
3 Cellometer software
Part II
Getting Started
12
................................................................................................................................... 131 Installation
.......................................................................................................................................................... 13Incoming inspection
.......................................................................................................................................................... 13
Software installation
.......................................................................................................................................................... 18
Take background image
................................................................................................................................... 202 Set up Cellometer Auto for cell counting
.......................................................................................................................................................... 20Prepare cell solution
.......................................................................................................................................................... 20
Load cells into counting chamber
.......................................................................................................................................................... 21
Insert chamber into Cellometer Auto
.......................................................................................................................................................... 21
Adjust focus
.......................................................................................................................................................... 22
Count cells
.......................................................................................................................................................... 22
Review results
.......................................................................................................................................................... 22
Recount Images
.......................................................................................................................................................... 22
Save data
.......................................................................................................................................................... 22
Save images
.......................................................................................................................................................... 22
Analysis
Part III
Software Features
24
................................................................................................................................... 251 Cellometer software panel
.......................................................................................................................................................... 26Buttons and controls
.......................................................................................................................................................... 26
User input
.......................................................................................................................................................... 26
Output
................................................................................................................................... 272 Cellometer software menu items
.......................................................................................................................................................... 27File
.......................................................................................................................................................... 27
Cell type
.......................................................................................................................................................... 29
Options
.......................................................................................................................................................... 31
Analysis
.......................................................................................................................................................... 33
Help
Part IV
Operation Reference
36
................................................................................................................................... 371 Background image
................................................................................................................................... 37
2 Exposure adjustment
................................................................................................................................... 37
3 Manual adjustment for cell counts
................................................................................................................................... 37
4 Set up new cell type
................................................................................................................................... 38
5 Speed Count
................................................................................................................................... 38
6 Count new samples