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Dead cells with a brighter center

Dead cells of this type have small bright centers, as shown in the following figure for CHO cells.

Dead cell parameter "Sensitivity" is modified to pick up this type of dead cell.

CHO cell counting results with different "sensitivity" settings. (a). CHO cell image, (b) to (e) sensitivity of the dead cell parameter

was increased incrementally from 1 to 4, respectively. Green outlines indicate live cells while red outlines indicate dead cells. Both

live and dead cell counts in (e) agree with visual observation of cell image in (a).

CHO cell parameters with high "Sensitivity" settings.