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5. Enter in dilution factor

6. Count cells

7. Output displays "Live Cell Concentration" (cells/ml), and "Viability" (%)

8. Check "Counted"

Live cells are indicated by green outlines, and dead cells are indicated by red outlines, as seen in the figure (b)

below.

MCF7 cell viability measurement using Auto T4: (a) MCF7 cell image and (b) counted MCF7 cells.