User's Manual

i
®
Model No. PS-2108 Dissolved Oxygen Sensor
Table of Contents
Equipment List .......................................................... 1
Introduction ............................................................. 1
DO Sensor Setup and Calibration...................................... 3
Prepare the Sensor for First Use ....................................................................................................3
Set up the Dissolved Oxygen Sensor with DataStudio..................................................................3
Equilibrate the Probe in 100% Humidified Air .............................................................................4
Perform a Single-point Calibration (mg/l Dissolved O2)..............................................................4
Setup to Calibrate in Percent (%) Saturation.................................................................................4
General Sampling Procedure............................................ 6
Measurements in a Controlled Laboratory Setting........................................................................6
Using the Dissolved Oxygen Sensor with other PASCO Sensors:................................................6
Maintenance ............................................................. 7
Changing the Electrolyte solution..................................................................................................7
Replacing the Membrane...............................................................................................................7
Replacing the O-ring......................................................................................................................8
Storage .................................................................. 9
Short-Term Storage (two weeks or less)........................................................................................9
Long-Term Storage (more than two weeks)..................................................................................9
Troubleshooting ......................................................... 9
Theory of Dissolved Oxygen: ......................................... 10
What is the chemical mechanism by which diatomic oxygen dissolves in water?......................10
Citations:......................................................................................................................................14
Experiment 1:
Introduction to the Operation of the Dissolved O2 Sensor ........ 15
Purpose.........................................................................................................................................15
Materials and Equipment Needed................................................................................................15
Optional Equipment Suggested ...................................................................................................15
Procedure .....................................................................................................................................15
Data Analysis/Questions..............................................................................................................16
Experiment 2:
Photosynthesis and Oxygen Generation with Aquatic Plants ........ 17
Purpose.........................................................................................................................................17
Materials and Equipment Needed................................................................................................17
Procedure .....................................................................................................................................18
During the lab time ......................................................................................................................18
Collecting Data ............................................................................................................................19
Sample Data.................................................................................................................................19
Analysis .......................................................................................................................................19