User's Manual

Ocean Optics with Xplorer GLX Appendix: Beer's Law Procedure

Appendix: Beer's Law Procedure

Follow these steps to obtain an absorbance versus concentration graph

for aqueous solutions of copper(II) sulfate (CuSO

Equipment

You will need the following items:

Set-up

1. Turn on the GLX.

2. Connect the spectrometer to the GLX.

Result: The Initializing screen appears, followed by the

Configuration screen.

3. Attach the Integrated Light Source and Cuvette Holder to the spec-

trometer.

4. Fill a cuvette with your weakest solution sample (this would typi-

cally be just water).

5. Place the cuvette in the cuvette holder oriented (as illustrated) so

that light travels through the cuvette by the shorter path.

6. In the Data Acquisition tab of the Configuration screen, set

Integration Time to 25 ms.

7. Check the spectrum preview in the upper part of the screen; it

should not be clipped, especially in the region of 800 nm. If it is,

reduce the integration time.

8. Press to switch to the Lamp tab of the Configuration screen.

9. With the cuvette in place, and the lamp on (you should see light in

the cuvette), press (Save Ref).

10. Press to switch the lamp off.

11. Press (Save Dark).

12. Press to switch the lamp back on.

Xplorer GLX (with Ocean Optics license key installed)

Ocean Optics Spectrometer

Integrated Light Source and Cuvette Holder

Cuvettes (included with above item)

Various aqueous solutions of CuSO

of known concentrations

ranging between 0 and 0.5 M. About 3 mL of each sample

needed.

Cuvette