User's Manual

Ocean Optics with Xplorer GLX Measurements

2. Press to begin recording data.

3. Press again to stop recording. The data run is saved with a

name such as “Run #1 - 500.0 nm” to indicate the value of the

Wavelength parameter.

Note: See the GLX Users' Guide for general information about collecting, display-

ing and analyzing data on the GLX and uploading data to a computer.

Beer's Law Mode

Use Beer's Law mode to make a graph of Absorbance versus Concentra-

tion. To use this mode, you will need an integrated light source and

cuvette holder, cuvettes, and several different known concentrations of a

solution. Beer's Law mode is similar to Time Acquisition mode in that

data is collected from a single channel defined by the Wavelength and

Bandwidth parameters. Follow the procedure on page 9 to use Beer’s

Law mode.

Measurements

In addition to the simple Intensity measurement, the GLX records mea-

surements derived from Intensity, the dark scan, and the reference scan.

(If you have not saved dark and reference scans, the GLX will not

record derived measurements.)

To select a measurement for display in the Graph screen:

1. Press to “light up” the Graph's active fields, with the “Y-axis”

measurement highlighted.

2. Press again to open the menu.

3. Use the arrow keys to highlight the desired measurement and

press to select it.

The measurements are described below.

Intensity

This is the straight measurement made by the spectrometer in units of

digital counts.

Minus Dark

The GLX calculates Minus Dark by subtracting the dark scan from the

Intensity measurement.

Beer’s Law mode

Graph screen menu containing

measurements