Use Instructions

Instructions For Use
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IFU-3M001-EN-00 (2021.03.05)
LOAA(Lab on An Array) Real-Time PCR Instrument
5. Instructions for Use of LOAA Analyzer
Note Cartridge preparation may vary depending on the cartridge type. For specific preparation steps, refer to the
Instructions for Use for each Dr. PCR cartridge kit.
There are three main steps to take when using LOAA Analyzer system.
1) Inject and dispense the sample mixture into cartridge using LOAA Sample Loader (Model Name: POSTMAN).
2) Install the Cartridge to the LOAA Analyzer and operate the device.
3) Check the PCR results.
5.1. Dr. PCR Cartridge Kit
Each kit contains the reagents and cartridges needed for PCR test to be performed in LOAA Analyzer. Components vary
depending on the kit type. Please refer to the Instructions For Use or Quick Guide for specific preparation and test
procedures.
Note. Cartridge may vary depending on the kit type.
Caution:
Do not use the components of one LOAA cartridge kit to prepare another type of LOAA cartridge. Components
are specific to each LOAA cartridge.
5.2. Preparation before PCR test
5.2.1. General precautions
It is recommended that the handling of potentially infected samples be carried out in a biological safety cabinet or hood, or
behind a protective shield. Overall procedure starts with DNA/RNA extraction and purification using commercially available
prep kit. Recommended samples for LOAA Diagnosis are nucleic acids extracted from whole blood, plasma and serum.
The mixed solution is loaded into cartridge by using sample loader. Finally, the sample loaded cartridge is inserted in the
analyzer and starts test. The detail steps starting from the sample loading is addressed below.
One of the most important guidelines related to tests using PCR is to prevent contamination.
The followings are some of important rules.
1) Sample collection, cartridge injection, cartridge loading and LOAA Analyzer device operation must be performed
in separate locations or work areas.
2) Do not leave the work area or return to the previous work area without completing the decontamination procedure
at first (cleaning the area and changing protective clothes and gloves).
3) Prepare only one cartridge at a time.
4) Always dispose of used cartridges or cartridges in contact with samples in biological hazardous waste containers.
Replace gloves after handling used cartridges.
LOAA cartridges are stored in individually packed pouches. Always check the availability and operation of LOAA Analyzer
device before removing the individual package to install the cartridge.
5.2.2. Preparation of the LOAA Analyzer and LOAA Sample Loader
LOAA Analyzer and LOAA sample loader should be ready for PCR test by following the operation steps described in 3.4.