Use Instructions

Instructions For Use
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IFU-3M001-EN-00 (2021.03.05)
LOAA(Lab on An Array) Real-Time PCR Instrument
3. Operating Principles
LOAA Analyzer is an in vitro diagnostic system that diagnoses targets in the clinical sample by using multiplex real-time
PCR to synthesize cDNA from nucleic acids extracted from whole blood, plasma, serum or urine, and to amplify the
synthesized cDNA as template.
Users of LOAA Analyzer system install the cartridge to the LOAA Sample Loader POSTMAN, inject the extracted nucleic
acid sample to the cartridge, place it on the device and start the test.
LOAA Analyzer interacts with a cartridge to amplify the RNA or DNA sequence of the pathogens in the sample under
examination. The resultant PCR products are automatically measured and analyzed by LOAA Analyzer to display the test
results.
3.1. Basics of PCR
Polymerase chain reaction (PCR) is a process that produces billions of copies of DNA. If the target is RNA, reverse
transcription is performed at 50°C to synthesize cDNA. Then DNA double strand is split into two single strands at 95°C and
the probe is bound to the specific gene sequence at 60°C. While duplicating a specific gene sequence, the fluorescence
level will increase and it is measured in real time. While temperature cycle changes alternately between 95°C and 60°C,
fluorescence measurement is performed periodically at the specific step during each cycle.