Use Instructions

Instructions For Use
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IFU-3M001-EN-00 (2021.03.05)
LOAA(Lab on An Array) Real-Time PCR Instrument
Fluorescence intensity at each well is detected by the CMOS photosensor chip at each thermal cycle during PCR
amplification. The fluorescence intensity real-time curve is generated and analyzed by the LOAA software in the analyzer
to determine if targets are detected at each well.
1.3.1. LOAA Sample Loader (Model name: POSTMAN) and Dr. PCR Cartridge Interaction
Before starting the test, Dr. PCR cartridge should be loaded into LOAA Sample Loader (Model name: POSTMAN) in order
to deliver appropriated amount of sample solution into the well array. Put the liquid reagent prepared with the purified nucleic
acid and PCR reagents into the cartridge injection port using a disposable pipette. When you press the activating button on
the LOAA Sample Loader (Model name: POSTMAN), the sample mixture automatically enters the individual well inside the
cartridge and the operation stops automatically when the injection is complete.
1.3.2. Interaction between LOAA Analyzer and cartridge
The mixture of purified nucleic acid and sample master mix amplifies both the control material and the target identified by
the cartridge. Cartridge and equipment perform reverse transcription and/or thermal cycling (heating and cooling of the
solution) for PCR reaction. Thermal cycle control parameters are set appropriately according to the associated test protocol
for each specific reagent cartridge and sample type.
1.3.3. PCR reaction analysis
To identify the target in a positive PCR reaction, LOAA performs a fluorescence amplification curve analysis. During each
PCR cycle, multiple images of PCR well array are captured and fluorescence levels of valid wells are calculated and
fluorescence amplification curve is generated at each PCR cycle by the software installed in LOAA. Based on this curve, if
it turns out to be a valid PCR reaction through PCR control reaction result analysis, PCR reaction result that which targets
are detected in the sample is delivered to user. Validation of proper operation of the optical system included in LOAA
Analyzer is monitored through device self-check operation and cartridge PCR control reactions.
1.4. LOAA Analyzer Software
LOAA Analyzer software provides the system controls the operation of LOAA Analyzer. The software also collects, analyzes,
and archives data generated by the device. The analysis results are presented in the test result table. Detailed information
on the features and operation of LOAA Analyzer software is provided in Chapter 5.