User Manual

17
TROUBLESHOOTING GUIDE
Problem
Cause
Remedy
Caution
Laser beam output
No laser beam is output.
The laser oscillator is not ON.
Set the main switch and key of the laser oscillator to ON.
Never look into an objective to check if the laser beam is output.
The illumination selector knob is set for the mer- cury burner illuminator.
Push in the illumination selector knob to select the laser illuminator light path.
The filter slider of the fiber light illuminator is pulled out.
Push in the filter slider to open the light path.
The manual shutter of the fluorescence mirror unit cassette is closed.
Release the manual shutter.
Other fluorescence mirror unit than a total internal reflection fluorescence mirror unit is engaged in the light path.
Engage a total internal reflection fluorescence mirror unit in the light path.
The laser beam incidence angle adjustment knob is turned too much counterclockwise.
Turn the laser beam incidence angle adjustment knob all the way clockwise.
The binocular tube shutter is pulled out.
Push in the binocular tube shutter.
Classification
It takes long time after the laser oscillator is switched ON till the laser beam is output.
The equipment is normal if the laser beam is out- put within about 1 minute.
Wait until the laser beam is output.
The interlock key switch is set to OFF.
Set the interlock key switch to ON.
The AC adapter connector is connected im- properly.
Laser beam observation
Total internal reflection fluorescence cannot be obtained.
The laser incidence angle is not the critical angle.
Turn the laser incidence angle adjustment knob counter clockwise.
The laser beam incidence angle adjustment knob is turned too much counterclockwise.
Turn the laser beam incidence angle adjustment knob clockwise.
An objective other than the total internal reflection fluorescence objective is engaged in the light path.
Engage a total internal reflection fluorescence objective in the light path.
Be sure to pulled out the shut- ter to block the laser beam be- fore proceeding to treatment.
The specimen is tilted.
Place the cover glass so that it is parallel with the stage center plate.
The specimen being observed has a refractive index of 1.38 or more (glycerin sealed specimen, etc.)
Use the Apo100XOHR objective (NA 1.65) to observe a speci- men with a refractive index of 1.38 (index of cytoplasm) or more.
The immersion oil or cover glass is not suitable.
Special immersion oil and cover glass are required when using the Apo100XOHR (NA 1.65).
Linear or elliptical interference stripes are observed in the field.
The ND filter in the filter slider is tilted.
Stand the ND filter vertically using the ring spring provided in the filter slider. If the interference stripe still does not disappear, rotate the ND filter to minimize the interference stripe.
Be sure to pulled out the shut- ter to block the laser beam be- fore proceeding to treatment. If the interference stripe does not disappear after treatment, please consult Olympus.
A fluorescence mirror unit incorporating an excita- tion filter is engaged in the light path.
Engage a total reflection fluorescence mirror unit without excitation filter in the light path.
Random interference stripe is observed in the field, and the stripe moves when the stage is moved.
The specimen (cell) is not in close contact with the cover glass but is separated from it at some positions.
Put the specimen (cell) in close and perfect contact with the cover glass.
The immersion oil contains bubbles.
Wipe off the immersion oil and attach new oil by avoiding bubble penetration.
Random interference stripe is observed in the field, and the stripe does not move even when the stage is moved.
The objective is dirty. Clean the objective top lens with the cleaning mixture fluid.
Connect it properly.
The specimen replacement lid is removed.
Place the specimen replacement lid on the specimen box.
An interference stripe with a concentric pat- tern is observed.
Dust is attached to the dichroic mirror or barrier filter.
Blow dust away using a blower, etc.