Modules described in this manual IX2-RFAEVA-2 FV5-LAAR IX2-COMB FV5-FUR INSTRUCTIONS TOTAL INTERNAL REFLECTION ILLUMINATION SYSTEM This instruction manual is for the Olympus Total Internal Reflection Illumination System. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this system, we recommend that you study this manual thoroughly before operating the system. This system employs a laser light source.
This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive. NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
IX2-RFAEVA-2 CONTENTS CAUTION This system employs a laser light source and should be assembled and adjusted by qualified service personnel from Olympus. Never attempt to assemble or adjust the system for it is extremely hazardous. IMPORTANT – Be sure to read this section for safe use of the equipment. – 1-7 1 MODULE NOMENCLATURE 2 CONTROLS 9, 10 3 TOTAL INTERNAL REFLECTION FLUORESCENCE OBSERVATION 11-14 8 3-1 Conditions of Observation ..........................................................
IMPORTANT This system has been designed to be used in combination with an IX2 series (IX81/IX71/IX51) or IX series (IX70/IX50) microscope to provide it with total internal reflection fluorescence illumination* using a laser light source.
IX2-RFAEVA-2 Safety Symbols The following symbols are found on the system. Study the meaning of the symbols and always use the equipment in the safest possible manner. Symbol Explanation Indicates the use of a laser beam. Take special care in handling the part. Indicates presence of a high voltage (1 kV or more). Take care against electric shock. Indicates that the surface becomes hot, and should not be touched with bare hands. Before use, carefully read the instruction manual.
Warning Labels Warning labels are placed at parts where special precaution is required when handling and using the system. Always heed the warnings. If warning labels become soiled, peel off, etc., contact your local Olympus representative to have them replaced. Warning Label Positions }Warning labels are attached to the positions indicated by the arrows.
IX2-RFAEVA-2 CAUTION-CLASS 3B LASER RADIATION WHEN OPEN. AVOID EXPOSURE TO THE BEAM.
CAUTION-CLASS 3B LASER RADIATION WHEN OPEN. AVOID EXPOSURE TO THE BEAM. Microscope frame (rear right) AVOID EXPOSURE LASER RADIATION IS EMITTED FROM THIS APERTURE.
IX2-RFAEVA-2 SAFETY PRECAUTIONS Movement of the System The system is assembled and set up by qualified service personnel. Avoid moving the system for this may deviate the optical adjustments. Consult Olympus when you wish to move the system. If the system is moved by the customer, Olympus will not assume liabilities for the system malfunctions and damage that may result. Disposal of the System 1.
1 Getting Ready 1. This manual pertains only to the handling of the total internal reflection fluorescence illumination system. Please also read the instruction manuals for the IX2 series (IX series) microscope and associated options to understand the comprehensive operating instructions of the microscope system. 2. The total internal reflection fluorescence illumination system is composed of precision instruments. Handle it with care and avoid subjecting it to sudden or severe impact. 3.
IX2-RFAEVA-2 1 MODULE NOMENCLATURE ? The modules enclosed ( system. ) belong to the IX2-RFAEVA-2 total internal reflection fluorescence illumination For IX2/IX series: · Fluorescence mirror unit cassette · Fluorescence mirror unit (for mercury burner) · Fluorescence mirror unit (for laser)* Specimen Cover * Remove the excitation filter from each laser wavelength compatible excitation mirror unit * Remove the excitation filter from the IB before use. (See the chart on page 11.
CONTROLS Straight Light Illuminator and Fiber Light Illuminator Laser emission indicators Illumination selector knob Field iris diaphragm (FS) centering screw ON : Laser being emitted OFF : Shuttered : Laser light path : Mercury burner light path 1 IX2 series adapter 2 Laser interlock key switch ON : Interlocked OFF : Laser beam blocked Field iris diaphragm (FS) lever Filter slider Pushed in: Filter engaged Pulled out: Shutter engaged Laser incidence angle adjustment knob View in the direction of a
IX2-RFAEVA-2 Specimen Cover ?Lighting the specimen replacement lid activates the interlock key switch and the motorized shutters (x 2) of the fiber light illuminator blocks the laser beam.
TOTAL INTERNAL REFLECTION FLUORESCENCE OBSERVATION Because this observation method employs laser beam, be sure to observe the following precautions. · Never remove a part on which a warning panel is attached. · Be sure to set the interlock key switch to OFF and make sure that the laser emission indicator is OFF before proceeding to the objective switching or fluorescence turret switching. 3-1 Conditions of Observation Be sure to observe the following laser operating environment conditions.
IX2-RFAEVA-2 3-2 Preparation for Observation Never peel off warning label from a position it is attached. Otherwise, the laser beam may enter your eyes. @ 1 (Fig. 1) 1. Set the light path selector knob of the microscope for the camera light path. 2. Push in the illumination selector knob @ for the laser light path. 3. Rotate the fluorescence mirror unit turret to engage an excitation mirror unit that is compatible with total internal reflection fluorescence observation. 4.
3-3 Observation Procedure 1 @ Adjusting the Focus (Fig. 3) 1. Remove the specimen replacement lid @ from the stage. 2. Apply immersion oil on the objective top lens and place the specimen on the stage. 3. Place the specimen replacement lid @ on the specimen box ²; the laser beam will be output. When the image is displayed on the monitor for the TV camera, bring the displayed specimen image into focus. ² Fig. 3 2 @ Fig. 4 Adjusting the Laser Beam Incidence Angle (Fig.
IX2-RFAEVA-2 Explanation Diagrams (Laser Beam Incidence Angle Adjustment) Explanation 1 : Condition before laser incidence angle adjustment Glass Specimen Monitored image Field iris diaphragm image Immersion oil Objective Fluorescence image of specimen Laser beam · The laser beam is transmitting through the glass and specimen. · The fluorescence image of specimen is observed even when it is defocused by moving the objective upward.
REFLECTED LIGHT FLUORESCENCE OBSERVATION (Observation Using the Mercury Burner) }Follow the instructions given by the instruction manual for the reflected light fluorescence system in use. For this observation, be sure to attach the original fluorescence mirror unit, which incorporates the excitation filter, to the turret. USING THE CONTROLS 1 Fig. 5 2 ² Fig. 6 15 (Fig.
IX2-RFAEVA-2 3 Field Iris Aperture Diaphragm (Fig. 7) }To prevent progress of specimen fading, stop down the field iris aperture diaphragm to cover only the observed area. · The field iris diaphragm can be stopped down by pulling out the field iris diaphragm lever @. @ ³ Fig. 7 Centering the Field Iris Diaphragm (Figs. 7 & 8) 1. Place a specimen on the stage and bring the specimen image displayed on the monitor into focus. 2.
17 Laser beam observation Classification Laser beam output Turn the laser beam incidence angle adjustment knob all the way clockwise. Push in the binocular tube shutter. Place the specimen replacement lid on the specimen box. Set the interlock key switch to ON. The interlock key switch is set to OFF. The AC adapter connector is connected im- Connect it properly. properly. The equipment is normal if the laser beam is out- Wait until the laser beam is output. put within about 1 minute.
IX2-RFAEVA-2 SPECIFICATIONS Item Specification Applicable microscope frames · IX2 series (IX81/IX71/IX51) · IX series (IX70/IX50) Applicable reflected fluorescence systems · IX2 and IX series systems (Reflected light illuminator unnecessary) Total internal reflection illumination system IX2-RFAEVA Straight (mercury burner)/Fiber light (laser) double illuminator · Either illuminator can be selected using the illumination selector knob. · Interlock mechanism (Laser interlock/User interlock).
PROPER SELECTION OF THE POWER SUPPLY CORD If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “Specifications” and “Certified Cord” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment. Specifications 125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area) 6A minimum 60°C minimum 3.05 m maximum Grounding type attachment plug cap.
IX2-RFAEVA-2 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Approval Organization Alternative Marking Utilizing Printed or Embossed Harmonization Black-Red-Yellow Thread (Length Marking (May be located on jacket of color section in mm) or insulation of internal wiring) Black Red Yellow Comité Électrotechnique Belge (CEBEC) CEBEC 10 30 10 VDE Verband der Elektrotechnik Elektronik Informationstechnik e.V.
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