CONFOCAL LASER SCANNING BIOLOGICAL MICROSCOPES FV300 FLUOVIEW
FV300 CONFOCAL LASER SCANNING MICROSCO PERFORMANCE FOR THE PERSONAL USER The FV300 is the ideal choice of laser scanning microscopes for personal users. Its optical system is fully integrated, from scanner to microscope, and not only delivers outstanding optical sectioning, but also ensures the easy, flexible expandability required for any future upgrade.
OPE q w e r !2 !0 o !1 y u i t q Optical fiber for laser introduction w Beam collimator e Polarizer r Dichromatic mirror t Excitation dichromatic mirror y XY galvanometer mirror scanners u Pupil lens i Collector Lens o Pinhole turret !0 Emission beam splitter slider !1 Barrier filter slider !2 Photo multiplier 2
Software Graphical User Interface Ultimate ease of operation and monitor display. Dye selection display When a fluorescence dye is chosen, the laser and light path settings are selected automatically, with each of the selected fluorescence dyes displayed graphically on the monitor. Versatile display options Exchange between condensed and full image display modes can be performed with a single touch. Individual panel layouts can be changed at will, and the panel in use can be placed in any desired position.
Easy exchange between display methods Independent navigation bars for each image window enable the display method to be changed quickly and easily. Image tool bar X-Y-Z scanning operations and timelapse observations both produce multiple images, which can be displayed in sequence simply by clicking the sequential mode button. Channel selection and image zooming are also available on the same menu. Thumbnail display Data stored in the gallery window are displayed as thumbnails for easy viewing.
Time Course Using different scanning modes to chart time-lapse changes efficiently. X -t Y -t X-Y-t X-Z-t X-Y-Z-t ZoomIn-Z-t SlantLine-t FreeLine-t Y-Z-t ZoomIn-t X-Y-t Calcium wave in Xenopus oocyte, Calcium Green staining, fluorescence pseudo-colored fluorescence image after injection of inositol 3-trisphospate Japan Science and Technology Corporation, Exploratory Research for Advanced Technology, Mikoshiba cell control project, Prof.
FRET Hardware and software support to optimize the environment for FRET. The 440nm diode laser can be added for CFP/YFP FRET imaging. A 440nm diode laser is optionally available for CFP/YFP imaging. The 440nm laser line ideally excites CFP, with minimal disturbance to YFP, and is therefore suitable for CFP/YFP FRET experiments. The high performance LSM objectives, PLAPO40XWLSM and PLAPO60XWLSM, are precisely corrected in this wavelength range, and ensure the highest measuring reliability.
PAPP for FRAP Application PAPP: Programmable Acquisition Protocol Processor Easy, reliable flow of experiments for fluorescence recovery after photobleaching. Mouse; hippocampal neurons; fluorescence of GFP Living neurons expressing GFP were maintained in culture and fluorescent images were obtained. Subsequently, FRAP analysis was performed on the same cell to determine the diffusion rate of GFP proteins into the dendritic spines. Rapid fluorescence recovery ( within seconds ) was observed.
Multi-point time lapse system High-magnification multi-point time lapse observation of living cells. Wide-ranging specimen observations for improved experiment throughput Use of a motorized XY stage allows the analysis of time lapse changes in many points scattered over a wide area. The system is therefore effective for work with thick specimens, such as observing changes in the states and movements of stem cells using a brain slice, or analyzing expression mechanisms at the individual level in an embryo.
3D Imaging Using multiple 3D images to obtain accurate 3D structure analysis. X-Y-Z ZoomIn-Z X -Z Y -Z X-Y-Z SlantLine-Z FreeLine-Z Easy Z axis operation and setting The upper and lower limit of Z scanning can be specified interactively by actually scanning the sample or by direct input of the numerical value. Tile display Y -Z Acquire X-Y-Z images and display X-Y cross-sectional images quickly and continuously in increments of 0.01*µm Thanks to the precision driving mechanism that enables 0.
Colocalization Analyzing the degree of intensity overlap between channels. Colocalization By using this function to analyze multi-color specimens, it is possible to discover whether different labeled substances are present in the same region. The ability to quantify the Pearson correlation, the overlapping coefficient and the colocalization index allows colocalization volumes to be compared between different specimens. Images can also be analyzed in series.
Applications Gallery Neuron Lucifer Yellow: retina ganglion cell TexasRed: dopamine-operated amacrine cell Prof. Shigetada Nakanishi Dept. of Biological Sciences, Kyoto Univ. Faculty of Medicine Mouse hippocampal neurons GFP: postsynaptic density protein Rhodamine-phalloidin: actin Hippocampal neurons expressing a GFP-tagged postsynaptic density protein were fixed and stained with rhodamine-phalloidin to visualize the localization of cytoplasmic actin filaments.
nt e c s e r o u l F Proteins GFP-labeling of Drosophila adult brain with staining of mushroom bodies Assistant Prof. Aigaki Cytogenetics Tokyo Metropolitan University, Science Dept. Expression of DsRed in a zebrafish embryo Extended focus image of 5µmx30 slice Pr. Yasuhiro Kamei, Pr. Shunsuke Yuba Institute for Molecular and Cellular Biology Osaka University C elegans expressing beta-integrin fused to GFP Dr. Xioping Xhu and Dr. John Plenefisch University of Toledo, Dept.
Fluorescence Dyes and Filters 0 44 LD lti 8 45 Mu 400 300 e eN dH 3 63 Re 700 BA530RIF BA550RIF BA650RIF Short Pass 5 40 LD n go Ar Ne on on on rgon / e g g r A Ar ti A nH ton lti l 8 ee yp 48 Mu Kr Gr Mu 8 3 5 8 56 54 51 48 600 500 g Ar BA430-460 Band Pass BA465-495 BA470-520 BA480-495 BA480-510 BA505-525 BA505-550 BA510-540 BA520-550 BA535-565 BA540-590 BA560-600 BA585-615 BA585-640 BA510IF Long Pass BA560IF BA565IF BA585IF BA590 BA610IF BA625IF BA660IF RD M5 SD 530 M6 SD
Specifications Laser light source Scanning unit Item Specifications Visible light laser source Select from the following laser, to mounted on laser combiner Multi-line Ar laser (458nm, 488nm, 515nm, Total 40mW), Ar laser (488nm,10mW), Kr laser (568nm, 10mW), HeNe (G) laser (543nm,1mW), HeNe (R) laser (633nm,10mW), LD405 (405nm, 25mW), LD440 (440nm, 5.
1130 FV300-IX dimensions 1200 2330 (unit: mm) 1310 1310 1500 (unit: mm) 1370 FV300-BX dimensions 1130 1200 2330 Depth: 990 Different types of laser combiners Depth: 990 External transmitted light detector and fluorescence illumination system Selectable from ND filter or AOTF combiner. The shutters and light intensity can be controlled via the Fluoview computer. * Laser combiner for AOTF is required for multi-line Argon laser.
