Use Instructions

21 Additional calibrations for a fluorescence system
7. Click the [Next] button to start the shading correction.
8. Continue in a similar way with the 10x objective.
9. For the 10x objective, go to the 100x/60x checkerboard area and defocus
by going down 40µm.
10. Click the [Finish] button to finalize the shading correction.
21.9 Shading correction for PhaseContrast (PH)
Prepare a slide with some sample on it for focusing. The rest of the slide should
be empty and very clean.
1. In the [Calibrations] dialog box select the [Shading correction] entry.
2. Click the [Calibrate] button.
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Instructions for useOLYMPUS VS200 System