Use Instructions

21 Additional calibrations for a fluorescence system
8. Select an area on the slide large enough for a 2x field of view and focus the
sample via ‘Ctrl’ + mouse wheel and proceed with [Next].
For objectives equal or higher than 10x try to find a sample area
which is quite homogenous.
9. Repeat these steps for all other selected objectives.
10. Continue the shading correction for all other fluorescence observation
methods.
ATTENTION
In case you have calibrated an immersion medium objective as well clean the
objective and remove immersion medium residues from the calibration slide.
See Immersion Objectives auf Seite 38.
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Instructions for useOLYMPUS VS200 System