Use Instructions

21 Additional calibrations for a fluorescence system
3. If you are calibrating an out of the box system (no shading correction was
done before) start with the acquisition of the dark current correction
image.
4. After the dark current image calibration select all objectives. Immersion
objectives are calibrated in the same wizard. However the calibration wiz-
ard will calibrate all dry objectives first and subsequently select the immer-
sion medium objective(s).
5. Make sure the [Shading correction] check box is selected
6. Proceed with [Next].
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Instructions for useOLYMPUS VS200 System