Use Instructions

21 Additional calibrations for a fluorescence system
mand.
21.1 Camera adapter
The parfocality might change due to temperature changes. Therefore
do not perform this calibration with a “cold” system (e.g. directly after
assembling the system). Wait at least 30 min with the system switched
on to heat it up.
If you are using objectives with a cover slip correction collar, it is recom-
mended to adjust it prior to all subsequent calibrations.
The limit for the focus distance is +/- 20µm. However the closer you get
to zero the better.
21.1.1 Camera adapter U-FFWO T3
This example describes the calibration for an ORCA Flash 4.0. The cal-
ibration for the VS-304M as well as the ORCA Fusion VS200 can be done in
the same way.
Small hex key to lock the tubus
Hex key to open the filter wheel
Small spanner to adjust the height of the tubus
Spanner to fix the tubus
The standard for the focus distance is +/- 20μm. However the closer you
get to zero the better.
1. In the [Calibrations] dialog box select the [Camera Adapter] entry and click
the [Calibrate] button.
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Instructions for useOLYMPUS VS200 System