Operating Manual and User’s Guide S2000 Miniature Fiber Optic Spectrometers and Accessories Offices: East Coast 380 Main Street, Dunedin, Fla., USA 727.733.2447 • 727.733.3962 Fax 8:30 a.m.-6 p.m. EST West Coast 5190 Golden Foothill Parkway, El Dorado Hills, Calif., USA 916.939-4300 • 916.939.4307 Fax Noon-9 p.m. EST Ocean Optics B.V. (Europe) Nieuwgraaf 108 G, 6921 RK DUIVEN, The Netherlands 31-(0)26-3190500 • 31-(0)26-3190505 Fax E-mail: Info@OceanOptics.
Copyright © 2000 Ocean Optics, Inc. All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or stored in a retrieval system, without written permission from Ocean Optics, Inc. This manual may be saved on the customer's PC and may be printed in sufficient quantities for those using and operating Ocean Optics products only and not for resale.
Table of Contents Introduction ..........................................................................................................................5 Quick Start .............................................................................................................................9 S2000 Miniature Fiber Optic Spectrometer ......................................................11 S2000 Specifications...................................................................................................
Table of Contents Fiber Optic Probes and Accessories ...................................................................83 R200 Reflection Probes .......................................................................................................84 RPH-1 Reflection Probe Holder ...........................................................................................85 T300-RT-UV/VIS Transmission Dip Probe...........................................................................
Introduction Ocean Optics miniature fiber optic spectrometers and accessories have revolutionized the analytical instrumentation market by dramatically reducing the size and cost of optical sensing systems. More than 15,000 Ocean Optics spectrometers have been sold worldwide -- striking evidence of the far-reaching impact of lowcost, miniature components for fiber optic spectroscopy.
Introduction OOIBase32 Spectrometer Operating Software is our next generation of operating software for all Ocean Optics spectrometers. OOIBase32 is a 32-bit, user-customizable, advanced acquisition and display program that provides a real-time interface to a variety of signal-processing functions for Windows 95/98, Windows 2000 and Windows NT users.
Introduction Packing List A packing list comes with your order. It is located inside a plastic bag attached to the outside of the shipment box. The invoice is mailed separately. The items listed on your packing slip include all of the components in your order. However, some items on your packing list are actually items you have specified to be installed into your spectrometer, such as the grating, detector collection lens and slit.
Quick Start The S2000 is easy to set up, allowing the user to start collecting data within minutes. The three pages in this section provide brief instructions on setting up your system, installing your A/D converter, installing and configuring the software, and connecting sampling optics. If you prefer step-by-step directions for setting up and operating any part of your system, check the Table of Contents to find instructions on a specific component.
Quick Start 4. Note these values. When you first run OOIBase32, you must enter these values in the “Configure Hardware” dialog box. If your A/D converter is the SAD500 If your A/D converter is the SAD500 and is mounted onto the spectrometer, connect the cable from the SAD500 to your PC. If you ordered your SAD500 in its own housing, attach another cable from the spectrometer to the SAD500. Note the serial port number (also called COM Port) on the PC to which you are interfacing.
Quick Start Configure Data Acquisition Dialog Box Finally, select Spectrum | Configure Data Acquisition from the menu to open the Configure Data Acquisition dialog box and to set your data acquisition parameters such as the integration time, averaging and boxcar smoothing, and several other parameters.
S2000 Miniature Fiber Optic Spectrometers Our second-generation miniature fiber optic spectrometer, the S2000, couples a low-cost, high-performance 2048-element linear CCD-array detector with an optical bench that's small enough to fit into the palm of your hand. The S2000 is a high-sensitivity, low-cost UV-VIS-Shortwave NIR spectrometer, making it especially useful for fluorescence and other low light level applications that demand high detector sensitivity.
S2000 Miniature Fiber Optic Spectrometers S2000 Specifications Absolute Maximum Ratings VCC Voltage on any pin + 5.5 VDC VCC + 0.2 VDC Physical Specifications Physical dimensions (no enclosure) Physical dimension (with enclosure) Weight 5.47” x 3.90” x 0.75” LWH (master only) 139 mm x 99 mm x 19 mm LWH (master only) 5.63” x 4.09” x 1.
S2000 Miniature Fiber Optic Spectrometers S2000 Board Layout H1 H2 JP3 JP4 JP5 JP6 RV1 RV2 J1 J2 Header Block connecting analog signals from slave spectrometer channel(s) to the Master spectrometer channel Header Block containing digital control signals Jumper Block controlling of external strobing of a light source Jumper Block controlling externally triggered integration time of the spectrometer Reserved -- do not short Reserved -- do not short Potentiometer controlling the baseline Potentiometer contr
A/D Converters This section covers the basic installation instructions for our A/D converters: ADC500, ADC1000 (and PC2000), SAD500, and DAQ700. Because A/D converter installation goes hand-in-hand with software installation, you will find directions for installing OOIBase32 Spectrometer Operating Software included in this section.
A/D Converters: ADC500 ADC500 ISA-bus A/D Converter The ADC500 ISA-BUS A/D CONVERTER is a 12-bit, 8-channel ISA-bus analog-to-digital converter card that connects our spectrometers to desktop PCs. This single-ended, half-length card fits into an ISA slot in a desktop PC, and has a 500 kHz sampling frequency. The following are directions for installing your ADC500.
A/D Converters: ADC500 Windows NT Users: Find Available Base Address and IRQ Settings 1. 2. 3. 4. 5. Go to Start | Programs | Administrative Tools (Common) | Windows NT Diagnostics. In the “Windows NT Diagnostics” dialog box, click on the Resources tab. Select the IRQ button. Find an available IRQ -- a number unassigned to a device. Select the I/O Port button. Find an available I/O Range (Base Address) -- a number or range of numbers unassigned to a device. (The number is in hexadecimal.
A/D Converters: ADC500 Run OOIBase32 After you restart your computer, navigate to the OOIBase32 icon and select it. The first time you run OOIBase32 after installation, you must follow several prompts before you can begin taking measurements. Operator and Serial Number Dialog Box First, a prompt to enter a user name and serial number appears. Certain data files will include this information in the header.
A/D Converters: ADC500 Spectrometer Configuration Dialog Box Now that OOIBase32 is running, you need to configure your system. Select Spectrometer | Configure from the menu. Go through each page in the Spectrometer Configuration dialog box to set system parameters. (See the OOIBase32 Spectrometer Operating Software Manual for details.) " In the Wavelength Calibration page, the coefficients for each spectrometer channel in your system have already been loaded as part of the spectrometer configuration file.
A/D Converters: ADC1000 (and PC2000) ADC1000 ISA-bus A/D Converter (and PC2000) The ADC1000 ISA-BUS A/D CONVERTER is a 12-bit, 8-channel, single-ended A/D card that connects our spectrometers to desktop PCs. The PC2000 PC PLUG-IN SPECTROMETER has our 2048-element linear CCD-array fiber optic spectrometer mounted onto an ADC1000. This sturdy combination fits easily into a slot in the PC. The following are directions for installing your ADC1000 and PC2000.
A/D Converters: ADC1000 (and PC2000) Windows NT Users: Find Available Base Address and IRQ Settings 1. 2. 3. 4. 5. Go to Start | Programs | Administrative Tools (Common) | Windows NT Diagnostics. In the “Windows NT Diagnostics” dialog box, click on the Resources tab. Select the IRQ button. Find an available IRQ -- a number unassigned to a device. Select the I/O Port button. Find an available I/O Range (Base Address) -- a number or range of numbers unassigned to a device. (The number is in hexadecimal.
A/D Converters: ADC1000 (and PC2000) Run OOIBase32 After you restart your computer, navigate to the OOIBase32 icon and select it. The first time you run OOIBase32 after installation, you must follow several prompts before you can begin taking measurements. Operator and Serial Number Dialog Box First, a prompt to enter a user name and serial number appears. Certain data files will include this information in the header.
A/D Converters: ADC1000 (and PC2000) Spectrometer Configuration Dialog Box Now that OOIBase32 is running, you need to configure your system. Select Spectrometer | Configure from the menu. Go through each page in the Spectrometer Configuration dialog box to set system parameters. (See the OOIBase32 Spectrometer Operating Software Manual for details.
A/D Converters: SAD500 SAD500 Serial Port Interface The SAD500 SERIAL PORT INTERFACE is a microprocessor-controlled A/D converter for serial port connection or stand-alone operation. The SAD500 can be used to interface to desktop or portable PCs, PLCs, and other devices that support the RS-232 communication protocol. The following are directions for setting up your SAD500.
A/D Converters: SAD500 Configure Hardware Dialog Box Next, the Configure Hardware dialog box opens. The parameters in this dialog box are usually set only once -- when OOIBase32 is first installed and the software first runs. 1. Under Spectrometer Type, choose your spectrometer. 2. Under A/D Converter Type, choose SAD500. 3. Under Serial Port, choose the COM port number your computer is using to interface to your SAD500. See the Troubleshooting section to determine the COM Port. 4.
A/D Converters: SAD500 Save the spectrometer configuration file by choosing Spectrometer | Save Configuration As from the OOIBase32 menu. You can rename the file or use the default file name ([your serial number].spec). You will then be asked if you would like to make this file the default spectrometer configuration file. Choose Yes. The next time you run OOIBase32, the software will use the file as the standard for your configuration.
A/D Converters: DAQ-700 DAQ-700 PCMCIA A/D Converter The DAQ-700 PCMCIA A/D CONVERTER is a 12-bit analog-to-digital converter card that connects our spectrometers to notebook PCs. This 16-channel single-ended, 8-channel differential card -- also known by its National Instruments DAQCard-700 designation -- fits into a credit card-size slot in a notebook PC. It has a 100 kHz sampling frequency.
A/D Converters: DAQ-700 For Windows 95/98 Users: Configure the DAQ-700 1. 2. 3. 4. 5. 6. 7. If you hear the “happy” sound, click Start, and select Settings | Control Panel. Double-click the System icon. Select the Device Manager tab. In the “Device Manager” dialog box, find the hardware group named Data Acquisition Devices. Either double-click the group or select the group and click Properties. Under the Data Acquisition Devices group, find the entry for your DAQ-700.
A/D Converters: DAQ-700 8. 9. While making this change, notice the Conflict information area at the bottom. Make sure you choose a value that says No devices are conflicting. If it shows a conflict, you must select a different value. After selecting values with no conflicts, click OK. You will then see the “Creating a Forced Configuration” message box. Click Yes. Note your values of both the Input/Output Range (Base Address) and the Interrupt Request (IRQ).
A/D Converters: DAQ-700 Run OOIBase32 After you restart your computer, navigate to the OOIBase32 icon and select it. The very first time you run OOIBase32 after installation, you must follow several prompts before you can begin taking measurements. Operator and Serial Number Dialog Box First, a prompt to enter a user name and serial number appears. Certain data files will include this information in the header.
A/D Converters: DAQ-700 Spectrometer Configuration Dialog Box Now that OOIBase32 is running, you need to configure your system. Select Spectrometer | Configure from the menu. Go through each page in the Spectrometer Configuration dialog box to set system parameters. (See the OOIBase32 Spectrometer Operating Software Manual for details.) " In the Wavelength Calibration page, the coefficients for each spectrometer channel in your system have already been loaded as part of the spectrometer configuration file.
OOIBase32 Spectrometer Operating Software OOIBASE32 SPECTROMETER OPERATING SOFTWARE is our next generation of operating software for all Ocean Optics spectrometers. OOIBase32 is a 32-bit, user-customizable, advanced acquisition and display program that provides a real-time interface to a variety of signal-processing functions for Windows 95/98 and Windows NT users.
OOIBase32 Spectrometer Operating Software Other advanced features provide you with several data collection options. You have the ability to independently store and retrieve dark, reference, sample and processed spectra. All data can be saved to disk using autoincremented filenames. You can save data as ASCII files or in the native GRAMS/32 SPC format. One feature prints the spectra and another copies spectral or graphical data into other software such as Microsoft Excel and Word.
Light Sources Ocean Optics light sources provide illumination and excitation in the UV, VIS or Shortwave NIR range for absorbance, reflection and fluorescence measurements; discrete line spectra for wavelength calibrations; and calibrated absolute intensity standards for irradiance measurements. Wall transformers are supplied for laboratory use. Most of the light sources are powered by +12 VDC to facilitate their use in the field.
Light Sources: MINI-D2T MINI-D2T Miniature Deuterium Tungsten Light Source The MINI-D2T MINIATURE DEUTERIUM TUNGSTEN LIGHT SOURCE combines the continuous spectrum of an RFcoupled deuterium UV light source and a tungsten halogen VIS/Shortwave NIR light source in a single optical path. The combined-spectrum light source produces a peak-to-peak stability of 0.3% from ~200-850 nm.
Light Sources: MINI-D2T 4. 5. 6. When you want to control the MINI-D2T through OOIBase32 Spectrometer Operating Software, select or deselect the Strobe Enable box in the Acquisition Parameter dialog bar above the graph area to turn the light source in the MINI-D2T on and off. There can be up to a 1.5 second delay between turning the bulbs on via the software and the bulbs igniting. If the lamp has not been used recently, the deuterium bulb could take up to 60 seconds to ignite. For 0.
Light Sources: D-1000 D-1000 Deuterium Light Source The D-1000 DEUTERIUM LIGHT SOURCE is a high-output, high stability fiber optic light source optimized for the UV. The D-1000 produces intense, continuous spectral output from ~200-400 nm, making it especially useful for UV spectroscopy. The D-1000 has a highly stabilized microprocessor-based power supply designed for optimum stability, with a maximum fluctuation of just 0.05% peak-to-peak and drift of +/- 0.5%/hr.
Light Sources: D-1000 8. 9. Lift up the black protective shutter and disconnect your optical fiber from the SMA connector. Replace the red plastic cap over the SMA connector. Bulb Replacement 1. 2. 3. Order a replacement bulb, item D-1000-B, from Ocean Optics. Make sure the D-1000 is turned off, the power cord is disconnected and the lamp has cooled. Use a Phillips-head screwdriver to remove all 12 screws from the side panels of the D-1000 casing.
Light Sources: DT-1000 DT-1000 Deuterium Tungsten Halogen Light Source The DT-1000 DEUTERIUM TUNGSTEN HALOGEN LIGHT SOURCE combines the continuous spectrum of a deuterium UV light source and a tungsten-halogen VIS/Shortwave NIR light source in a single optical path. The combined light source produces a powerful, stable output from ~200-1100 nm. It also has a highly stabilized microprocessor-based power supply designed for optimum stability.
Light Sources: DT-1000 Operating the Deuterium Source 1. 2. To power the deuterium lamp, push in the UV Start button located on the front panel. This white button turns on the deuterium lamp. Pushing in the UV Start button initiates the start-up sequence for the lamp. First, the heater in the deuterium lamp ionizes the available deuterium. At this point, the yellow Heater On light on the front panel will light. After ~30 seconds, the red UV On light located at the top of the front panel will light.
Light Sources: DT-1000 4. 5. Gently grip the top of the bulb and pull it away from the cylindrical unit. Discard the bulb. Insert the new bulb into the top of the cylinder, being careful to position the bulb’s pins over the holes in the top of the cylinder. ! The cylindrical unit is held in place with a small set screw. By loosening it, the user can slide the bulb unit up and down, positioning it in front of the attenuator, a metal disc that attenuates the light before going through the collimating lens.
Light Sources: LS-1 LS-1 Tungsten Halogen Light Source The LS-1 TUNGSTEN HALOGEN LIGHT SOURCE is a versatile, white-light lamp utilized for the VIS-Shortwave NIR (360 nm-2 µm). The lamp, available with either a 900-hour or 10,000-hour bulb, offers high color temperature and extremely efficient output. Parts Included " " " LS-1 Tungsten Halogen Light Source 12 VAC power supply Allen wrench for adjusting the collimating lens Caution! # # DO NOT insert plastic or flammable materials into the filter slot.
Light Sources: LS-1 4. Locate the two set screws at the back of the lamp, one above each back leg. These two screws keep the two halves of the lamp together. Remove the two screws. 5. Gently separate the two halves of the lamp. 6. Carefully pull the bulb out of its housing. Detach the wire and lamp leads from the socket. Remove the old bulb unit and discard. 7. Plug the new bulb into the socket. 8. Slide the new bulb forward into the front of the lamp as far as it will go. 9.
Light Sources: PX-2 PX-2 Pulsed Xenon Lamp The PX-2 PULSED XENON LAMP is a high flash rate, short-arc xenon lamp for applications involving absorbance, reflection, fluorescence and phosphorescence measurements. The PX-2 operates at speeds up to 220 Hz and offers critical pulse-to-pulse stability.
Light Sources: PX-2 operating software.
Light Sources: LS-450 LS-450 Blue LED Pulsed Light Source The LS-450 BLUE LED PULSED LIGHT SOURCE is a compact, low-cost light-emitting diode that produces pulsed or continuous spectral output at 470 nm -- the blue region -- for high-sensitivity emission fluorescence measurements. The LS-450 excitation source can be combined with other sampling optics for fluorescence applications. Operation 1. 2. Plug the wall transformer into a standard 110V outlet and into the back of the LS-450.
Light Sources: R-LS-450 R-LS-450 Rack-mount Blue LED Pulsed Light Source The R-LS-450 BLUE LED PULSED LIGHT SOURCE is a compact, low-cost light-emitting diode that produces pulsed or continuous spectral output at 470 nm -- the blue region -- for high-sensitivity emission fluorescence measurements. The R-LS-450 is the rack mount version of the LS-450. The R-LS-450 can be configured to operate in continuous wave mode through manual operation and through the software.
Light Sources: R-LS-450 Jumper Block 3 (JP3) There are two sets of pins in JP3. The jumper position here determines the source of control for the R-LS-450: manual or remote control. A jumper over the Remote pins means that you can control the R-LS-450 through the software (if other jumper blocks are configured correctly). R-LS-450 Operating Matrix This matrix will help you configure the jumper blocks on the R-LS-450.
Light Sources: R-LS-450 You can control the pulses per second of the R-LS-450 through the Flash Delay function in the OOIBase32 software if: " The switch is turned to pulsed mode " There is a jumper over the pins in JP1 on the R-LS-450 board " There is a jumper over the pins labeled CS in JP2 on the R-LS-450 board " There is a jumper over the pins labeled Remote in JP3 on the R-LS-450 board " There is a jumper over the pins labeled 2 in JP3 on the S2000 board Setting the Integration Time When using any of
Light Sources: HG-1 HG-1 Mercury Argon Calibration Source The HG-1 MERCURY ARGON CALIBRATION SOURCE is a wavelength calibration source for UV-VIS-Shortwave NIR spectrophotometric systems. The HG-1 produces Mercury and Argon lines from 253-922 nm, for use in performing fast, accurate spectrometer wavelength calibrations. The HG-1 has an SMA 905 termination for connecting to optical fibers. Caution! # # The beam emerging from the HG-1 produces ultraviolet radiation.
Light Sources: HG-1 1. 2. 3. After placing OOIBase32 into Scope Mode, take a spectrum of your light source. Adjust the integration time until there are several peaks on the screen that are not off-scale. Move the cursor to one of the peaks and carefully position it so that it is at the point of maximum intensity. Record the pixel number that is displayed in the status bar (located beneath the graph). Repeat this step for all of the peaks in your spectrum.
Light Sources: HG-1 6. 7. 8. The numbers of importance are indicated in the above figure. You will need to record the Intercept as well as the First, Second, and Third Coefficients. Also, look at the value for R squared. It should be very close to 1. If it is not, you have probably assigned one of your wavelengths incorrectly. Select Spectrometer | Configure from the menu and choose the Wavelength Calibration page to update the wavelength coefficients within OOIBase32.
Light Sources: LS-1-CAL LS-1-CAL Calibrated Light Source The LS-1-CAL CALIBRATED LIGHT SOURCE for the VIS-Shortwave NIR (300-1050 nm) is a tungsten halogen light source that provides you with known absolute intensity values at several wavelengths, expressed in µW/cm2/nm.
Light Sources: LS-1-CAL 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. Find the on/off switch on the back of the lamp and turn the lamp on. Let the lamp warm up for at least 15 minutes before using. Insert the disk that came with your lamp into your computer. The disk contains two ASCII files. These files have the same information as the Lamp Calibration Reports that came with your LS-1-CAL.
Sampling Chambers Ocean Optics offers a comprehensive line of compact, low-cost sampling chambers for a variety of UV-VISShortwave NIR applications. All sampling chambers have SMA terminations for easy coupling to optical fibers. Options include a variety of cuvette holders for 1-cm and 10-cm cuvettes, in-line filter holders, flow cells, and other sampling devices. " CUV-UV and CUV-VIS CUVETTE HOLDERS, are our standard cuvette holders for 1-cm cuvettes.
Sampling Chambers: CUV-UV, CUV-VIS CUV-UV, CUV-VIS Cuvette Holders The CUV-UV and CUV-VIS CUVETTE HOLDERS for 1-cm path length cuvettes couple via SMA-terminated optical fibers to our spectrometers and light sources to create small-footprint spectrophotometric systems for absorbance and transmission experiments. These holders can be optimized for UV-VIS-NIR (~200-1100 nm) or VIS-NIR (~360-1100 nm) applications.
Sampling Chambers: CUV-UV, CUV-VIS Specifications Path length: Collimating lenses (VIS-NIR): 1 cm BK 7 glass (~360 nm - 2 µm*), 5 mm diameter, f/2 Collimating lenses (UV-VIS-NIR): Collimating lens termination: Collimating lenses assembly (sample compartment) dimensions: Filter slot: Base material: Base length: Dynasil 1100 quartz (200 nm-2 µm*), 5 mm diameter, f/2 SMA 905 Connections in base: Water input fittings: Typical optical fibers specified for optimum performance (light throughput and optical re
Sampling Chambers: CUV-UV-10, CUV-VIS-10 CUV-UV-10, CUV-VIS-10 Cuvette Holders The CUV-UV-10 and CUV-VIS-10 CUVETTE HOLDERS for 10-cm cuvettes couple to our spectrometers and light sources to create spectrophotometric systems for absorbance and transmission measurements of aqueous solutions and gases for UV-VIS-NIR (~200-1100 nm) or VIS-NIR (~360-1100 nm) applications.
Sampling Chambers: CUV-ALL CUV-ALL 4-way Cuvette Holder The CUV-ALL 4-WAY CUVETTE HOLDER for 1-cm path length cuvettes has four collimating lenses that couple to optical fibers, light sources, and spectrometers to measure absorbance, fluorescence, scattering, or any combination of these optical phenomena.
Sampling Chambers: CUV-ALL Installing Filters 1. 2. 3. Loosen the filter clamping screw with the provided screwdriver. Insert the filter into the filter slot. The filter slot can accommodate filters up to 6 mm thick. Clamp the filter in place by gently tightening the clamping screw finger tight. Using the Temperature Stabilization Feature This feature is used to heat or cool the cuvette holder base and cuvette. 1. Remove the two plugs from the top side of the base.
Sampling Chambers: CUV-FL-DA CUV-FL-DA Direct Attach Cuvette Holder The CUV-FL-DA DIRECT ATTACH CUVETTE HOLDER attaches directly to our light sources and couples via SMA-terminated optical fibers to our spectrometers, creating an incredibly small-footprint spectrophotometric system for fluorescence as well as absorbance experiments. The CUV-FL-DA, optimized for UV-VIS-NIR (200-1100 nm) applications, holds 1-cm square cuvettes. The CUV-FL-DA is especially useful for fluorescence measurements.
Sampling Chambers: CUV-FL-DA Specifications Path length: Dimensions: Material: Hole diameter for mounting to light source: Collimating lens: Collimating lens termination: Filter slot: Reflection mirrors: Mirror diameter: Size of light beam reaching sample: 1 cm 1.4” x 1.4” x 1.0” (L x W x H) Black anodized aluminum 0.375” Dynasil 1100 quartz (200 nm - 2 µm), 5 mm diameter SMA 905 accepts filters up to ¼" (6 mm) in thickness UV-enhanced, aluminum-coated for optimal signal reflection and collection 0.3” (7.
Sampling Chambers: ISS ISS Integrated Sampling System The ISS INTEGRATED SAMPLING SYSTEM is a fully integrated 1-cm cuvette holder and tungsten halogen light source. It couples to our spectrometers with optical fiber to create a small-footprint systems for VIS-NIR (~360-1100 nm). The ISS has a 10,000 hour bulb and the fan is enclosed in the base.
Sampling Chambers: ISS 8. Tighten the set screw on the bottom of the lamp. 9. Close the two halves without pinching the wires. Replace the two screws at the back of the lamp. 10. Check the lamp output and adjust the focus of the SMA connector, if necessary. See Appendix B for instructions on adjusting the focus of your collimating lens.
Sampling Chambers: ISS-2 ISS-2 Integrated Sampling System The ISS-2 INTEGRATED SAMPLING SYSTEM is a fully integrated 1-cm cuvette holder and tungsten halogen light source. It couples to our spectrometers with optical fiber to create a small-footprint systems for VIS-NIR (~3601100 nm). The ISS-2 has a 900 hour bulb. Also, in the ISS-2, the fan is not enclosed in the base; it is exposed and should be handled with care.
Sampling Chambers: ISS-2 6. 7. 8. 9. 10. Gently separate the two halves of the lamp and pull the bulb out of its housing. Detach the wire and socket from the lamp leads. Remove bulb unit and discard. Plug the new bulb into the socket and slide it forward into the front of the lamp as far as it will go. Tighten the set screw on the bottom of the lamp to hold the bulb in place. Close the two halves of the lamp, being careful not to pinch the wires.
Sampling Chambers: ISS-UV-VIS ISS-UV-VIS Integrated Sampling System The ISS-UV-VIS INTEGRATED SAMPLING SYSTEM is a combination of a RF deuterium source with a tungsten bulb connected to a cuvette holder for 1-cm cuvettes. The cuvette holder attaches directly to the light source and has a 5-mm diameter f/2 collimating lens. The ISS-UV-VIS can be operated manually or through the software.
Sampling Chambers: ISS-UV-VIS Operating the ISS-UV-VIS through Software 1. 2. 3. 4. 5. 6. Connect the 15-pin accessory cable from the spectrometer to the ISS-UV-VIS. Find the switch on the back of the ISS-UV-VIS. There are three positions: On, Off, and Remote. For Software operation, move the switch to the Remote position. Moving the switch to the Remote position enables you to control the lamp through the software, whether you are using OOIChem or OOIBase32 software.
Sampling Chambers: FHS-UV, FHS-VIS FHS-UV and FHS-VIS In-line Filter Holders The FHS-UV and FHS-VIS IN-LINE FILTER HOLDERS are low-cost spectrophotometric accessories for fast, convenient absorbance/transmission measurements of optical and other filters.
Sampling Chambers: FHS-UV, FHS-VIS Specifications FHS-VIS Collimating lenses (VIS-NIR): FHS-UV Collimating lenses (UV-VIS-NIR): Collimating lens termination: Filter slot: Base material: * BK 7 glass (~360 nm - 2 µm*) 5 mm diameter f/2 Dynasil 1100 quartz (~200 nm - 2 µm*) 5 mm diameter f/2 SMA 905 accepts filters 1" round or any size >1/2" square, up to 1/4" (6 mm) in thickness aluminum Though the product can be used to 2 µm, it can be configured to "see" only to 1100 nm with our S2000 spectrometer.
Sampling Chambers: LPC LPC Long Pass Flow Cells LPC LONG PASS FLOW CELLS from Ocean Optics couple to the company's high-sensitivity fiber optic spectrometers and compact light sources via SMA terminations for simple, efficient measurement of low-volume or low-concentration (ppb-ppt) aqueous samples. LPC Long Pass Flow Cells are available in 1-meter, 5-meter and 10-meter path lengths for absorbance measurements.
Sampling Chambers: LPC Assessing if the LPC is Free of Particles Fluids need to be relatively particle-free. Particles larger than 20 µm can be trapped inside the tubing and can then block or scatter a significant amount of light. To rid the LPC of particles, follow these steps: 1. Pump the sample fluid through the LPC. 2. While in Scope Mode, save a dark spectrum with the light source off and a reference spectrum with the light source on. 3.
Sampling Chambers: CUV-CCE CUV-CCE Electrophoresis Sample Cell The CUV-CCE ELECTROPHORESIS SAMPLE CELL for chromatography and capillary electrophoresis is an optical fixture for measuring the UV absorbance of fluids in chromatography or capillary electrophoresis systems. The cell is attached on-line, i.e., the light is projected through the sides of fused silica tubing without violating the tube integrity. For this reason, there are no pressure limitations associated with the device.
Sampling Chambers: CUV-CCE Checking the Alignment 1. 2. 3. 4. With the spectrometer running, observe the signal in Scope Mode. When the optical window is properly positioned, you can see a full UV transmission through the cell. If the polyimide is in the optical path, you will see just the red end of the spectra. If this occurs, loosen the fittings and slide the sample tube to align with the window until you achieve the best signal (on both the wavelength and intensity axes).
Sampling Optics Ocean Optics offers numerous spectroscopic accessories. A short description for each accessory featured in this manual is listed below. " The 74-UV and 74-VIS COLLIMATING LENSES screw onto the end of SMA-terminated optical fibers and other sampling optics to convert divergent beams of radiation (light) into a parallel beam. " The 74-90-UV RIGHT ANGLE REFLECTOR for collimating lenses has a mirror located under its cap that reflects light from the collimating lens to 90°.
Sampling Optics: 74-UV, 74-VIS 74-UV, 74-VIS Collimating Lenses In order to obtain accurate data, the light entering the sample and the light collected after exiting the sample must be well collimated. The 74-UV and 74-VIS COLLIMATING LENSES screw onto the end of SMA-terminated optical fibers and other sampling optics to convert divergent beams of radiation (light) into a parallel beam. Application Tips " Using a collimating lens is easy.
Sampling Optics: 74-90-UV 74-90-UV Right Angle Reflector The 74-90-UV RIGHT ANGLE REFLECTOR is a 3/8-24 threaded black anodized aluminum assembly for mounting collimating lenses at right angles, and is useful for applications involving limited space and inconvenient optical fiber routing. Application Tips " " " The 74-90-UV Right Angle Reflector has a plane mirror located under its cap that reflects light from the collimating lens to 90°.
Sampling Optics: 74-OPM 74-OPM Optical Post Mount The 74-OPM OPTICAL POST MOUNT is a 3/8-24 threaded black anodized aluminum assembly used for mounting collimating lenses on breadboard laboratory tables, rail carriers and other bench plates. Application Tips " " " The 74-OPM Optical Post Mount has an 8-32 x ½" bore for mounting collimating lenses on breadboard laboratory tables, rail carriers and other bench plates. The 74-OPM is 1-1/2" in diameter and 0.
Sampling Optics: 74- ACH 74-ACH Adjustable Collimating Lens Holder The 74-ACH ADJUSTABLE COLLIMATING LENS HOLDER is a versatile assembly for mounting lenses at multiple positions, and is especially useful for transmission measurements of large or thick samples not easily accommodated by other sampling optics such as our FHS-UV and FHS-VIS In-line Filter Holders. Application Tips " " " " " The 74-ACH consists of an anodized aluminum base and adjustable mount bars.
Sampling Optics: FVA-UV FVA-UV Fiber Optic Variable Attenuator The FVA-UV FIBER OPTIC VARIABLE ATTENUATOR is an opto-mechanical device that controls the amount of light entering the optical bench of the spectrometer. Each end of the attenuator has SMA 905 terminations for connecting the attenuator to light sources, sample holders, and fibers. The FVA-UV attenuates light uniformly at all wavelengths.
Sampling Optics: WS-1 WS-1 Diffuse Reflectance Standard The WS-1 DIFFUSE REFLECTANCE STANDARD is a compact physical standard for use in performing diffuse reflectance measurements, especially color analysis. The reflectance material that comprises the WS-1 is Spectralon, a highly lambertian thermoplastic substance that provides a nearly 100% diffuse reflective surface for applications from 200 nm to 2.5 µm. (With an Ocean Optics spectrometer, the practical use of the WS-1 is limited to 200-1100 nm.
Sampling Optics: ISP-REF ISP-REF Integrating Sphere The ISP-REF INTEGRATING SPHERE is an illuminated sampling optic that couples via optical fiber to Ocean Optics miniature fiber optic spectrometers to measure reflectance of solid objects or emission of spectral sources. The ISP-REF Integrating Sphere has a transfer optic assembly for restricting the fiber viewing angle, a 0.4" aperture sample port, and a built-in light source (tungsten halogen) with 12-volt DC adapter.
Sampling Optics: FOIS-1 FOIS-1 Fiber Optic Integrating Sphere The FOIS-1 FIBER OPTIC INTEGRATING SPHERE is a sampling optic that accepts light energy through its 0.375" input port and funnels it to an optical fiber for emission experiments -- such as measuring the spectral properties of LEDs and other light sources. The FOIS-1 consists of a 1.5" Spectralon sphere encased in an aluminum housing, with a 0.
Fiber Optic Probes and Accessories Ocean Optics fiber optic probes couple to our miniature fiber optic spectrometers and light sources to create a variety of optical-sensing systems. Each probe consists of silica-core, silica-clad optical fiber (0.22 NA) and a sampling optic, and are available for UV-VIS (high OH content) or VIS-NIR (low OH content) applications. All standard fiber optic probes are 2 meters in length and have SMA terminations. Custom probe assemblies are also available.
Fiber Optic Probes and Accessories: R200 R200 Reflection Probes R200 REFLECTION PROBES couple to Ocean Optics miniature fiber optic spectrometers and light sources to create small-footprint optical-sensing systems for fluorescence and reflection measurements. Ocean Optics offers several variations on the Reflection Probe: " The R200-7 REFLECTION PROBE consists of a bundle of 7 optical fibers -- 6 illumination fibers around 1 read fiber -- each of which is 200 µm in diameter. A 3.0" x 0.
Fiber Optic Probes and Accessories: RPH-1 RPH-1 Reflection Probe Holder The RPH-1 REFLECTION PROBE HOLDER is an anodized aluminum platform with machined holes at 45° and 90° to hold our R200 Reflection Probes or other 0.25" O.D. probes during reflection measurements. Common applications include measuring the reflection properties of mirrors and anti-reflection coatings, and measuring the visual properties of color in paints, graphic arts, plastic, and food products.
Fiber Optic Probes and Accessories: T300 T300-RT-UV-VIS Transmission Dip Probe The T300-RT-UV/VIS TRANSMISSION DIP PROBE couples to our spectrometers and light sources to create smallfootprint optical-sensing systems for measuring in situ transmission in chemical solutions and other liquids. The standard T300-RT-UV/VIS Transmission Dip Probe has (2) 300 µm diameter solarization-resistant fibers (1 illumination, 1 read), in a 5.0" x 0.25" OD stainless steel ferrule.
Fiber Optic Probes and Accessories: CC-3 CC-3 Cosine-corrected Irradiance Probes The CC-3 COSINE-CORRECTED IRRADIANCE PROBES are spectroradiometric sampling optics designed to collect radiation (light) from a 180° field of view, thus eliminating light collection interface problems inherent to other sampling devices. The CC-3 COSINE-CORRECTOR (300-1000 nm) has glass diffusing material. The CC-3-UV COSINE-CORRECTOR (200 nm to 2 µm) has Teflon diffusing material.
Fiber Optic Probes and Accessories: FL-400 FL-400 Flame-resistant Fiber Probe The FL-400 FLAME-RESISTANT FIBER PROBE is a heat-resistant fiber optic probe that couples to Ocean Optics miniature fiber optic spectrometers to measure in situ emission spectra of samples such as dissolved metals and high-temperature plasmas. The FL-400 is a high-temperature 400-µm gold-jacketed UV-VIS optical fiber in an 8" long nickel sleeve. It can operate in environments up to 750º C.
Optical Fiber Assemblies Ocean Optics offers an extensive line of optical fibers and accessories -- including patch cords, bifurcated assemblies, bushings, and splitters -- for a variety of UV-VIS and VIS-NIR applications. All optical fibers couple easily via SMA terminations to our miniature fiber optic spectrometers, light sources and sampling optics.
Optical Fiber Assemblies Splice Bushings, Bulkhead Fittings Splice bushings are used to couple two SMA-terminated fibers together. Simply screw each fiber into the splice bushing finger-tight. Bulkhead fittings are used to fixture a fiber onto a panel. Install the bulkhead by drilling a hole in the wall where you wish to mount the fiber. Fasten with the lock washer and nut provided.
Experiment Tutorial Before you begin your experiment, double-check that you have correctly installed your A/D converter, installed the operating software, and set up your light source and other sampling optics. Next: 1. Open OOIBase32. Although you already configured your hardware when you installed your A/D converter, double-check that A/D Interface settings correspond to your setup by choosing Spectrometer | Configure from the menu. 2. Now check your spectrometer setup configurations in OOIBase32.
Experiment Tutorial: Absorbance Absorbance Experiments Absorbance spectra are a measure of how much light is absorbed by a sample. For most samples, absorbance is linearly related to the concentration of the substance. The software calculates absorbance (Aλ) using the following equation: Aλ = - log10 ( Sλ - Dλ Rλ - Dλ ) where S is the sample intensity at wavelength λ, D is the dark intensity at wavelength λ, R is the reference intensity at wavelength λ.
Experiment Tutorial: Transmission Transmission Experiments Transmission is the percentage of energy passing through a system relative to the amount that passes through the reference. Transmission Mode is also used to show the portion of light reflected from a sample. Transmission and reflection measurements require the same mathematical calculations. The transmission is expressed as a percentage (%Tλ) relative to a standard substance (such as air).
Experiment Tutorial: Reflection Reflection Experiments Reflection is the return of radiation by a surface, without a change in wavelength. The reflection may be: " Specular, in which the angle of incidence is equal to the angle of reflection. " Diffuse, in which the angle of incidence is not equal to the angle of reflection. Every surface returns both specular and diffuse reflections. Some surfaces may return mostly specular reflection, others more diffuse reflection.
Experiment Tutorial: Relative Irradiance Relative Irradiance Experiments Irradiance is the amount of energy at each wavelength from a radiant sample. In relative terms, it is the fraction of energy from the sample compared to the energy collected from a lamp with a blackbody energy distribution, normalized to 1 at the energy maximum.
Experiment Tutorial: Time Acquisition Time Acquisition Experiments Our OOIBase32 Spectrometer Operating Software allows you to perform time acquisition experiments. Time acquisition experiments track processes, perform kinetic analyses, and monitor spectral events as a function of time. You can collect as a function of time spectral data from up to 6 single wavelengths (designated as Channels A through F) and up to two mathematical combinations of these wavelengths (designated as Combinations 1 and 2).
Experiment Tutorial: Time Acquisition 7. Access the Time Acquisition Configuration dialog box by selecting Time Acquisition | Configure | Configure Acquisition from the menu. " Enable Stream Data to Disk to save time acquisition data. " Enter a value in the Write Data to Disk Every X Acquisitions box to set the frequency for saving data. The smaller this number, the more frequently data is saved.
Appendix A: Changing the A/D Converter Settings Base Address Settings for the ADC500 To change the Base Address settings on the ADC500 board, see the bank of 6 dip switches labeled SW1. Switches in the OFF position have the decimal values shown. Switches in the ON position have a value of zero. The Base Address is the sum of the values of the switches. In the default setting, switches 5 and 6 are added to give a total of 768. A few of the many combinations for Base Address settings are below.
Appendix A Interrupt Request Settings for the ADC500 To change the IRQ settings on the ADC500 board, see the bank of 4 dip switches labeled SW2. In the default setting, the IRQ is set to 7. Other combinations for IRQ settings are below. After you have changed the switches, reinstall the card and change the software settings to match the hardware settings. (See pages 15-18 for instructions.) The gray block indicates the position of the switch.
Appendix A Base Address Settings for the ADC1000 and PC2000 To change the Base Address settings on the ADC1000 and PC2000, see the bank of switches on the A/D board: The Base Address may be changed via the first 6 switches (the IRQ may be changed via the last 3 switches). Switches in the OFF position have the decimal values shown. Switches in the ON position have a value of zero. The Base Address is the sum of the values of the switches.
Appendix A Interrupt Request Settings for the ADC1000 and PC2000 To change the IRQ settings on the ADC1000 and PC2000, see the bank of switches on the A/D board: The IRQ may be changed via the last 3 switches. The following matrix defines the different IRQ settings by switch positions 7, 8, and 9. In the default setting, the IRQ is set to 7. Other combinations for IRQ settings are below. After you have changed the switches, reinstall the card and change the software settings to match the hardware settings.
Appendix B: Adjusting the Focus for Collimating Lenses In order to obtain accurate data, the light entering and exiting a sample by means of a fiber/collimating lens assembly must be well collimated. The following is a description of how to adjust the focus of light so that accurate data is collected by the spectrometer. (All collimating lenses are already adjusted at the time of manufacture such that light emerging from a 200 µm fiber/collimating lens assembly is collimated.
Appendix C: Calibrating the Wavelength of the Spectrometer The following describes how to calibrate the wavelength of your spectrometer. Though each spectrometer is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions. You are going to be solving the following equation, which shows that the relationship between pixel number and wavelength is a third-order polynomial . . . λp = I + C1 p + C2 p2 + C3 p3 . . .
Appendix C Independent Variable True Wavelength (nm) 253.65 296.73 302.15 313.16 334.15 365.02 404.66 407.78 435.84 546.07 576.96 579.07 696.54 706.72 727.29 738.40 751.47 4. 5.
Appendix D: S2000 Pin-outs and Jumpers The average user would not normally need to know about the interconnect scheme of the S2000, as the cables supplied with all of the units need only be plugged into the matching connectors on the hardware. However, if the need arises to design and fabricate your own cabling system, the following tables supply the necessary information.
Appendix D J2 (D-SUB-15) Accessory Connector J2 Pin DB-15 (Female) 1 Single Strobe 2 Continuous Strobe 3 VCC External Hardware Trigger External Synchronization Trigger Channel 7 Channel 6 4 5 6 7 8 D3 or External Software Trigger 9 10 11 12 Channel 1 GND Channel 4 Channel 5 13 S0 and S1 14 15 Channel 3 Channel 2 Description TTL output signal used to pulse a strobe that is high at the start of each integration period.
Appendix D H1 Header Pins (Analog) Pin 1 2 3 4 5 6 7 8 9 10 H2 Header Pins (Digital) Description Pin Analog Channel 0 Analog Channel 1 Analog Channel 2 Analog Channel 3 Analog Channel 4 Ground Reserved Analog Channel 7 Analog Channel 6 Analog Channel 5 D C B A 1 2 3 4 5 6 7 8 9 10 11 12 H1 and H2 Header Blocks connect a master unit to one or more slaves Description N/C A/D Trigger Digital In 3 (D3) S1 Ground +5 VDC Phi A/D clock Phi Read Out Gate Reserved Temperature (optional) Read Enable S0 Strobe
Appendix E: PC2000 Pin-outs and Jumpers The average user would not normally need to know about the interconnect scheme of the S2000, as the cables supplied with all of the units need only be plugged into the matching connectors on the hardware. However, if the need arises to design and fabricate your own cabling system, the following tables supply the necessary information.
Appendix F: External Triggering Our S2000 and S1024DW Spectrometers, when used with OOIBase32 Spectrometer Operating Software, provide four methods of acquiring data. In the Normal Mode, Ocean Optics spectrometers are “free running.” That is, the spectrometer is continuously scanning, acquiring, and transferring data to your computer, according to parameters set in the software. In this mode, however, there is no way to synchronize the scanning, acquiring and transferring of data with an external event.
Appendix F 2. 3. 4. 5. 6. source to supply the voltage, as it is based on a referenced ground and your reference may be different from ours. Using Pin 3 to supply voltage ensures that the spectrometer will receive the appropriate voltage for the trigger event. Supply a line from Pin 8 of the J2 Accessory Connector to your triggering device. (See figure on page 109 for pin location.
Appendix F 4. 5. 6. Configure Data Acquisition from the menu. Select Spectrum | Configure Data Acquisition from the menu. Choose the External Trigger page and select Synchronization. To save processed data with each external trigger, enable the Automatically save file on trigger box. If you enable this function, you will be presented with a file save dialog box with each trigger.
Appendix F 4. 5. Once you have selected the integration time, note the configuration of the pins in the Jumper Block 4 column of the chart. Remove your spectrometer from its housing. Do not tamper with the optical bench. (If you have more than one channel in your system, you may have to disconnect the channels from one another. The master spectrometer is usually on the bottom of a multiple channel system.) In the center of the green circuit board, near the optical bench, find Jumper Block 4, labeled JP4.