Near Infrared Spectrometer Installation and Operation Manual Document Number 016-70000-000-02-201301 Offices: Ocean Optics, Inc. World Headquarters 830 Douglas Ave., Dunedin, FL, USA 34698 Phone 727.733.2447 Fax 727.733.3962 8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST E-mail: Info@OceanOptics.com (General sales inquiries) Orders@OceanOptics.com (Questions about orders) TechSupport@OceanOptics.
Additional Offices: Ocean Optics Asia 666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai, PRC. 200336 Phone 86.21.5206.8686 Fax 86.21.5206.8686 E-Mail Sun.Ling@OceanOptics.com Ocean Optics EMEA Sales and Support Center Geograaf 24, 6921 EW DUIVEN, The Netherlands Phone 31-(0)26-3190500 Fax 31-(0)26-3190505 E-Mail Info@OceanOptics.eu Regional Headquarters Maybachstrasse 11 73760 Ostfildern Phone 49-711 34 16 96-0 Fax 49-711 34 16 96-85 E-Mail Sales@Mikropack.
Table of Contents About This Manual......................................................................................................... iii Document Purpose and Intended Audience.............................................................................. iii What’s New in this Document ................................................................................................... iii Document Summary .................................................................................................
Table of Contents Chapter 3: Troubleshooting .............................................................. 13 Overview ....................................................................................................................... 13 NIRQuest Spectrometer Connected to Computer Prior to Operating Software .............. 13 Windows Operating Systems ....................................................................................................
About This Manual Document Purpose and Intended Audience This document provides you with instructions to get your system up and running. In addition to the NIRQuest Spectrometer installation and operation instructions, this manual also includes information for locating the SpectraSuite installation instructions (see Product-Related Documentation below).
1: Introduction Product-Related Documentation You can access documentation for Ocean Optics products by visiting our website at http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate document from the available drop-down lists. Or, use the Search by Model Number field at the bottom of the web page. • Detailed instructions for SpectraSuite Spectrometer Operating Software are located at: http://www.oceanoptics.com/technical/SpectraSuite.pdf.
Chapter 1 Introduction Product Description A high-performance optical bench, low-noise electronics and various grating options make NIRQuest Spectrometers truly extraordinary. This new generation of small-footprint, near infrared spectrometers is available in four models that cover various ranges between 900 nm and 2500 nm and are ideal for applications ranging from analyzing moisture content in food and beverage products to analyzing trace metals in wastewater.
1: Introduction The NIRQuest Spectrometer can connect via the USB port to any PC that uses a Windows Me/2000/XP operating system and has spectrometer operating software installed and configured for use with your NIRQuest Spectrometer. Furthermore, the NIRQuest Spectrometer has a serial port for interfacing to PCs, PLCs, and other devices that support the RS-232 communication protocol.
1: Introduction Software SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers. It is a completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device, as well as any other manufacturer’s USB instrumentation using the appropriate drivers.
1: Introduction • 6 slit widths • Electrical Performance • 16 bit, 500KHz A/D converter • Integration times from 1 ms to 120 seconds (depending on spectrometer model) • Embedded microcontroller allows programmatic control of all operating parameters and standalone operation • USB 2.
1: Introduction • 2 to 3 Times Better Noise Performance. This permits integration for longer periods of time and detection of smaller changes in absorbance. The charts below show 3x noise improvement for the NIRQuest512 over the NIR512 at a 10-second integration time. • Considerably Better Signal-to-Noise Ratio (now >15000:1 at 100 ms).
1: Introduction Interface Options The NIRQuest Spectrometer has both USB and serial port connectors (with the use of an adapter), enabling you to connect the spectrometer to a desktop or notebook computer via a USB port or serial port. However, you must create custom software if using the serial port. SpectraSuite software is available if you are connecting via the USB port.
1: Introduction Passwords for software applications can be found on the back of the Software and Resources Library CD package. Other Accessories Available Visit us at www.OceanOptics.com for a complete list of products available for all of your spectroscopy needs.
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Chapter 2 Installing the NIRQuest Spectrometer Overview You must install the operating software application prior to connecting the NIRQuest Spectrometer to the PC. The Ocean Optics spectrometer operating software installs the drivers required for NIRQuest Spectrometer installation. If you do not install the software first, the system will not properly recognize the NIRQuest Spectrometer.
3:Troubleshooting ► Procedure 1. Install spectrometer operating software on the destination computer, and then reboot the system. 2. Plug the +5VDC wall adapter into an electrical outlet, then connect the power cord to the 2.5 mm power jack (older versions may have a 1.3 mm power jack) on the rear of the NIRQuest Spectrometer. 3. Locate the USB cable (USB-CBL-1) that came with the NIRQuest Spectrometer. 4.
3: Troubleshooting Configuring the NIRQuest Spectrometer If you have followed the previous steps for connecting NIRQuest in USB mode and started SpectraSuite, the spectrometer is already acquiring data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light intensity. This means the software and hardware are correctly installed.
3:Troubleshooting SpectraSuite NIRQuest Spectrometer Features Select Spectrometer | Spectrometer Features, then select the TEC tab to display the NIRQuest Spectrometer controls. Control Description Enable Fan Check this box to turn on the cooling fan. Enable ThermoElectric Control Check this box to enable (recommended setting for laboratory conditions) or uncheck to disable the thermoelectric cooler. Be sure to always turn on TEC power before applying USB power.
Chapter 3 Troubleshooting Overview The following sections contain information on troubleshooting issues you may encounter when using the NIR Spectrometer. NIRQuest Spectrometer Connected to Computer Prior to Operating Software Windows Operating Systems If you connected your NIRQuest Spectrometer to the computer prior to installing your Ocean Optics software application, you may encounter installation issues that you must correct before your Ocean Optics device will operate properly.
3:Troubleshooting Remove the Unknown Device from Windows Device Manager ► Procedure 1. Open Windows Device Manager. Consult the Windows operating instructions for your computer for directions, if needed. 2. Locate the Other Devices option and expand the Other Devices selection by clicking on the "+" sign to the immediate left. Note Improperly installed USB devices can also appear under the Universal Serial Bus Controller option. Be sure to check this location if you cannot locate the unknown device. 3.
3: Troubleshooting 3. Delete the OOI_USB.INF in the INF directory. If your computer is running either the Windows 2000 or XP operating system, you must also delete the OOI_USB.PNF file in the INF directory. 4. Navigate to the Windows | System32 | Drivers directory. 5. Delete the EZUSB.SYS file. 6. Reinstall your Ocean Optics application and reboot the system when prompted. 7. Plug in the USB device. The system is now able to locate and install the correct drivers for the USB device.
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Appendix A Calibrating the Wavelength of the NIRQuest Spectrometer This Appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions.
A: Calibrating the Wavelength of the Spectrometer • A light source capable of producing spectral lines. Ocean Optics’ AR-1 Argon lamp or HG-1 Mercury-Argon lamp are ideal for this purpose. If you do not have an AR-1 or HG-1, you will need a spectral line source that produces several (at least 4-6) spectral lines in the wavelength region of your spectrometer. If an order-sorting filter is installed in the NIRQuest, the XE-1 Xenon lamp is necessary to perform the wavelength calibration.
A: Calibrating the Wavelength of the NIR Spectrometer Independent Variable Dependent Variables Values computed from the regression output Pixel # 3 Predicted Wavelength Difference 30625 5359375 253.56 0.09 87616 25934336 296.72 0.01 312 97344 30371328 302.40 -0.25 313.16 342 116964 40001688 313.02 0.13 334.15 402 161604 64964808 334.19 -0.05 365.02 490 240100 117649000 365.05 -0.04 404.66 604 364816 220348864 404.67 -0.01 407.78 613 375769 230346397 407.78 0.
A: Calibrating the Wavelength of the Spectrometer 7. Record the Intercept as well as the First, Second, and Third Coefficients. Also, look at the value for R squared. It should be very close to one. If it is not, you have probably assigned one of your wavelengths incorrectly. Keep these values at hand. Saving the New Calibration Coefficients: USB Mode Wavelength calibration coefficients unique to each NIRQuest Spectrometer are programmed into an EEPROM memory chip on the NIRQuest Spectrometer.
A: Calibrating the Wavelength of the NIR Spectrometer 12. Repeat Step 12 for all of the new values. 13. Click on the Save All Values button to save the information, and then Exit the USB Programmer software. The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the NIRQuest Spectrometer.
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Appendix B Specifications How the Spectrometer Works Light passes through the NIRQuest Spectrometer through a fixed optical path. The optical bench of the NIRQuest Spectrometer has no moving parts that can wear or break; all the components are fixed in place at the time of manufacture.
B: Specifications NIRQuest Spectrometer Component Table The following table describes each component in the NIRQuest Spectrometer. Items marked with an asterisk (*) can be user-specified (consult an Applications Scientist for more information). 1 SMA Connector Secures the input fiber to the spectrometer. Light from the input fiber enters the optical bench through this connector. A dark piece of material containing a rectangular aperture, which is mounted directly behind the SMA Connector.
B: Specifications NIRQuest512 Specifications NIRQuest512 NIRQuest512-1.9 NIRQuest512-2.2 NIRQuest512-2.5 PHYSICAL Dimensions (mm): 182 x 110 x 47 Weight (kg): 1.
B: Specifications 3 Longpass filter : nd 3 2 Order filter : NIRQuest512 NIRQuest512-1.9 OF1-RG830 longpass NIR filter (optional) OF1-CGA1000 longpass NIR filter (standard) NIRQuest512-2.2 NIRQuest512-2.5 OF1-RG830 longpass NIR filter (optional) N/A Standard Collimating and focusing mirrors: Gold-coated for enhanced NIR reflectivity Fiber optic connector: SMA 905 to 0.
B: Specifications NIRQuest512 NIRQuest512-1.9 NIRQuest512-2.2 NIRQuest512-2.5 ELECTRONICS Power consumption USB power +5V, 0.5 A maximum; DC input jack +5V, 3 A maximum Data transfer speed: Full scan to memory every 5 ms with USB 2.
B: Specifications NIRQuest512 NIRQuest512-1.9 Wired Ethernet interface: NIRQuest512-2.2 NIRQuest512-2.5 Yes, with adapter Wi-Fi range: 25 meters in free space Web server: Works with common browsers 1 “Useable range” is defined in the context of the NIRQuest model’s detector response and its typical grating response. For example, the 512-element detector has response at 850 nm, but grating response begins at 900 nm.
B: Specifications NIRQuest256-2.1 NIRQuest256-2.
B: Specifications NIRQuest256-2.1 Corrected linearity: Estimated Peak Noise Equivalent Power (NEP) (default configuration) NIRQuest256-2.5 >99.8% >99.6% 5 pW 25 pW ELECTRONICS Power consumption USB power +5V, 0.5 A maximum; DC input jack +5V, 3 A maximum Data transfer speed: Full scan to memory every 5 ms with USB 2.
B: Specifications NIRQuest256-2.1 NIRQuest256-2.5 WIRELESS/ETHERNET INTERFACE (optional add-on accessory) Wireless (Wi-Fi) interface: Yes, with adapter Wired Ethernet interface: Yes, with adapter Wi-Fi range: 25 meters in free space Web server: Works with common browsers 1 “Useable range” is defined in the context of the NIRQuest model’s detector response and its typical grating response. For example, the 512-element detector has response at 850 nm, but grating response begins at 900 nm.
B: Specifications NIRQuest Spectrometer – Slit Size vs. Resolution and Throughput The following chart illustrates the effect that varying slit sizes have on NIRQuest512 Spectrometer resolution and throughput: 16 60 50 12 40 10 8 30 6 20 4 Relative Intensity Resolution (FWHM) 14 10 2 0 0 50 100 150 0 200 Slit Size (microns) NIRQuest Gratings The following tables show the NIRQuest gratings available for preconfigured (standard) setups and for all options.
B: Specifications NIRQuest Gratings – All Options Grating Intended Use Groove Density (lines/mm) Spectral Range* Blaze Wavelength Best Efficiency (>30%) NIR1 NIRQuest512-2.5 NIRQuest256-2.5 75 1600 nm 1700 nm 1075-2500 nm NIR2 NIRQuest 512-2.2 NIRQuest512-2.5 NIRQuest256-2.1 NIRQuest256-2.5 100 1200 nm 1600 nm 900-2050 nm NIR3 NIRQuest512 150 ~800 nm 1100 nm 900-1700 nm NIRQuest512-1.9 NIRQuest512-2.2 NIRQuest 512-2.5 NIRQuest256-2.1, NIRQuest256-2.
B: Specifications Grating Efficiency Curves The following graphs show grating efficiency only. System sensitivity is due to several factors, including detector response and grating efficiency.
B: Specifications Grating NIR2, 900 – 2050 nm, 100 l/mm, Blazed at 1600 nm Grating NIR3, 900 – 1700 nm, 150 l/mm, Blazed at 1100 nm 016-700000-000-02-201301 35
B: Specifications Grating NIR10, 750 – 2200 nm, 300 l/mm, Blazed at 1200 nm Grating NIR11, 980 – 2500 nm, 400 l/mm, Blazed at 1600 nm 36 016-700000-000-02-201301
B: Specifications Grating NIR12, 900 – 2500 nm, 500 l/mm, Blazed at 1370 nm Grating NIR13, 800 – 2500 nm, 600 l/mm, Blazed at 1200 nm 016-700000-000-02-201301 37
B: Specifications Grating NIR14, 900 – 1700 nm, 1000 l/mm, Blazed at 1310 nm NIRQuest Optical Resolution for Standard Setups The following table lists the optical resolution (FWHM) by slit width for standard (preconfigured) setups. Optical resolution will vary by grating range and slit size. Optical Resolution by Slit Width Slit NIRQuest5121 NIRQuest512-2.22 NIRQuest512-2.53 NIRQuest256-2.12 NIRQuest256-2.53 SLIT-10 ~2.0 nm ~3.0 nm ~4.1 nm ~6.7 nm ~8.3 nm SLIT-25 ~3.1 nm 4.7 nm ~6.3 nm ~7.
B: Specifications 30-Pin Accessory Connector Pinout The NIRQuest features a 30-pin Accessory Connector, located on the side of the unit as shown: Location of NIRQuest 30-Pin Accessory Connector 30-Pin Accessory Connector Pinout Diagram When facing the 30-pin Accessory Connector on the front of the vertical wall of the NIRQuest, pin numbering is as follows: USB Port 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 30-Pin Accessory Connector P
B: Specifications Pin # Function Input/Output Description 3 GPIO (2) Input/Output General purpose software-programmable, digital input/output (channel number) 4 N/A N/A Reserved 5 Ground Input/Output Ground 6 I C SCL Input/Output I C clock signal for communication to other I C peripherals 7 GPIO (0) Input/Output General purpose software-programmable, digital input/output (channel number) 8 I C SDA Input/Output I C data signal for communication to other I C peripherals 9 GPIO (1
B: Specifications Pin # Function Input/Output Description 20 Continuous Strobe Output TTL output signal used to pulse a strobe – Divided down from the master clock signal 21 SPI Chip Select Output SPI Chip/Device Select signal for communication to other SPI peripherals 22 GPIO (6) Input/Output General purpose software-programmable, digital input/output (channel number) 23 N/A N/A Reserved 24 N/A N/A Reserved 25 Lamp Enable Output TTL signal driven Active HIGH when the Lamp Enable
B: Specifications NIRQuest 15-Pin Accessory Cable Pinout Pin # Description Pin # Description 1 Single_strobe 9 GPIO-9 2 ContStrobe 10 GND_SIGNAL 3 V5_SW 11 SDA 4 ExtTrigIn 12 SCL 5 ExtTrigIn 13 LampEnable 6 GPIO-8 14 A_IN 7 A_OUT 15 GPIO-7 8 ExtTrigIn 42 016-700000-000-02-201301
Appendix C NIRQuest512-2.5 and NIRQuest256-2.5 Sensitivity When configuring a system for operation out to 2.5µm it is important that you consider the following details: • Short Integration Times: For the detector to be sensitive out to 2.5µm, the detector’s band gap energy must be small. Unfortunately, this raises the absolute level of the detectors dark signal. Typical dark signals at 400ms (NIRQuest512-2.5) and 135ms (NIRQuest256-2.5) are shown below.
C: NIRQuest512-2.5 and NIRQuest256-2.5 Sensitivity The figure below shows the relative intensity of a NIRQuest 512-2.5 and a NIRQuest256-2.5 Spectrometer with a 25µm slit directly coupled to an LS-1 Light Source with a 400µm fiber.
______________________________________________________________________________ Index 1 15-pin accessory cable pinouts, 42 3 30-pin accessory connector diagram, 39 part numbers, 41 pin definitions, 39 A accessories, 3, 7 Accessories, 11 accessory connector pinout, 39 Adobe Acrobat Reader, 6 B breakout box, 3, 7 C Calibrating, 17 calibration coefficients saving in USB mode, 20 CCD Detector, 24 collimating mirror, 24 component table, 24 configuring, 11 D document audience, iii purpose, iii summary,
Index S shipment contents, 6 slit, 24 SMA Connector, 24 software, 3 Software and Resources Library CD, 6 Specifications Detector, 28 Gratings, 34 Spectrometer, 28 SpectraSuite, 3, 12 Spectroscopic Accessories, 11 T TE Cooler, 3 46 troubleshooting Linux systems, 15 Mac systems, 15 Windows systems, 13 Troubleshooting, 13 U upgrades, iv USB-ADP-PC, 6 USB-CBL-1, 6 W Wavelength Calibration, 17 Wavelength Calibration Data File, 6 Wavelength Calibration Data Sheet, 6 what's new, iii 016-700000-000-02-20
