NIR Near Infrared Fiber Optic Spectrometers Installation and Operation Manual Document Number 197-00000-512-02-0707 Offices: Ocean Optics, Inc. World Headquarters 830 Douglas Ave., Dunedin, FL, USA 34698 Phone 727.733.2447 Fax 727.733.3962 8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST E-mail: Info@OceanOptics.com (General sales inquiries) Orders@OceanOptics.com (Questions about orders) TechSupport@OceanOptics.
Additional Offices: Ocean Optics Asia 666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai, PRC. 200336 Phone 86.21.5206.8686 Fax 86.21.5206.8686 E-Mail Sun.Ling@OceanOptics.com Ocean Optics B.V. (Europe) Geograaf 24, 6921 EW DUIVEN, The Netherlands Phone 31-(0)26-3190500 Fax 31-(0)26-3190505 E-Mail Info@OceanOpticsBV.com Copyright © 2001-2007 Ocean Optics, Inc. All rights reserved.
Table of Contents About This Manual .......................................................................................................... iii Document Purpose and Intended Audience.............................................................................. iii What’s New in this Document ................................................................................................... iii Document Summary................................................................................................
Table of Contents Remove Improperly Installed Files ............................................................................................ 12 Mac Operating Systems ............................................................................................................ 13 Linux Operating Systems .......................................................................................................... 13 Troubleshooting the Serial Port Configuration.................................................
About This Manual Document Purpose and Intended Audience This document provides you with instructions to get your system up and running. In addition to the NIR Spectrometer installation and operation instructions, this manual also includes information for locating the SpectraSuite installation instructions (see Product-Related Documentation below).
1: Introduction Product-Related Documentation You can access documentation for Ocean Optics products by visiting our website at http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate document from the available drop-down lists. Or, use the Search by Model Number field at the bottom of the web page. • Detailed instructions for SpectraSuite Spectrometer Operating Software are located at: http://www.oceanoptics.com/technical/SpectraSuite.pdf.
Chapter 1 Introduction Product Description The NIR (Near Infrared) Fiber Optic Spectrometer connects to a notebook or desktop PC via a USB port. It requires an external +5VDC power supply (USB-CBL-PS) to power the spectrometer’s highperformance InGaAs array detector. The NIR Spectrometer and the 16-bit A/D converter share a single housing, forming a small-footprint plug-and-play system.
1: Introduction About the Modular Approach Ocean Optics fiber optic spectrometer systems are based on low-cost, modular data acquisition principles. A typical NIR Spectrometer system contains four basic elements: • The NIR Spectrometer • The Ocean Optics spectrometer operating software • A light (excitation source) • A variety of sampling optics (depending on application need) The light (excitation source) sends light through an optical fiber to the sample.
1: Introduction Interface Options Because the NIR Spectrometer has both a USB and a serial port, you can connect the NIR Spectrometer to a desktop or notebook PC via a USB port or to a desktop or notebook PC via a serial port.
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Chapter 2 Installing the NIR Spectrometer Overview You must install the operating software application prior to connecting the NIR Spectrometer to the PC. The Ocean Optics spectrometer operating software installs the drivers required for NIR Spectrometer installation. If you do not install the software first, the system will not properly recognize the NIR Spectrometer.
2: Installation 4. Insert the square end of the cable into the rear of the NIR Spectrometer, and then insert the rectangular end into the USB port of the computer. If the software was installed prior to connecting the NIR Spectrometer, the Add New Hardware Wizard appears and installs the NIR Spectrometer drivers. If the drivers do not successfully install, or if you connected the NIR Spectrometer to the computer before installing the software, consult Chapter 4: Troubleshooting.
2: Installation Configuring the NIR Spectrometer The NIR spectrometer can be used with either SpectraSuite or OOIBase32 software when connected to the USB port. The configuration process differs, depending on whether you are running SpectraSuite or OOIBase32 software. Configuring the NIR Spectrometer in SpectraSuite If you have followed the previous steps and started SpectraSuite, the spectrometer is already acquiring data.
2: Installation If you do not have web access, you can retrieve this document from the Software and Technical Resources CD included with your spectrometer shipment. NIR Controls There are a number of controls for the NIR Spectrometer that are available in through the spectrometer operating software. The software differs as to where these controls are located.
2: Installation Control Description Enable Periodic Update Check this box to enable periodic update of the NIR detector temperature controls. Update Interval If you enabled periodic updates, enter the interval period (in seconds). Show in Status Bar Check this box to display the detector temperature in the status bar. NIR Toolbar in OOIBase32 The NIR toolbar in OOIBase32 allows you to control a variety of options on the NIR Spectrometer. These options are also applicable to the NIR256.
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Chapter 3 Troubleshooting Overview The following sections contain information on troubleshooting issues you may encounter when using the NIR Spectrometer. NIR Spectrometer Connected to Computer Prior to Operating Software Windows Operating Systems If you connected your NIR Spectrometer to the computer prior to installing your Ocean Optics software application (SpectraSuite or OOIBase32), you may encounter installation issues that you must correct before your Ocean Optics device will operate properly.
3:Troubleshooting Remove the Unknown Device from Windows Device Manager ► Procedure 1. Open Windows Device Manager. Consult the Windows operating instructions for your computer for directions, if needed. 2. Locate the Other Devices option and expand the Other Devices selection by clicking on the "+" sign to the immediate left. Note Improperly installed USB devices can also appear under the Universal Serial Bus Controller option. Be sure to check this location if you cannot locate the unknown device. 3.
3: Troubleshooting 3. Delete the OOI_USB.INF in the INF directory. If your computer is running either the Windows 2000 or XP operating system, you must also delete the OOI_USB.PNF file in the INF directory. 4. Navigate to the Windows | System32 | Drivers directory. 5. Delete the EZUSB.SYS file. 6. Reinstall your Ocean Optics application and reboot the system when prompted. 7. Plug in the USB device. The system is now able to locate and install the correct drivers for the USB device.
3:Troubleshooting Operating System Instructions 1. Select Start | Settings | Control Panel | System Windows 2000 2. Select the Hardware tab 3. Click the Device Manager button. Windows NT Select Start | Programs | Administrative Tools (common) NT Diagnostics 3. Double-click on the Ports (COM & LPT) option to display COM port numbers. Ensure that no warning icon appears next to the NIR Spectrometer’s COM port. 4. Verify that the NIR Spectrometer’s COM port is active.
Appendix A Calibrating the Wavelength of the NIR Spectrometer This Appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions.
A: Calibrating the Wavelength of the Spectrometer • A light source capable of producing spectral lines. Ocean Optics’ AR-1 Argon lamp or HG-1 Mercury-Argon lamp are ideal for this purpose. If you do not have an AR-1 or HG-1, you will need a spectral line source that produces several (at least 4-6) spectral lines in the wavelength region of your spectrometer. • The NIR Spectrometer. • An optical fiber (for spectrometers without a built-in slit, a 50-µm fiber works best).
A: Calibrating the Wavelength of the NIR Spectrometer Independent Variable Dependent Variables Values computed from the regression output Pixel # 3 Predicted Wavelength Difference 30625 5359375 253.56 0.09 87616 25934336 296.72 0.01 312 97344 30371328 302.40 -0.25 313.16 342 116964 40001688 313.02 0.13 334.15 402 161604 64964808 334.19 -0.05 365.02 490 240100 117649000 365.05 -0.04 404.66 604 364816 220348864 404.67 -0.01 407.78 613 375769 230346397 407.78 0.
A: Calibrating the Wavelength of the Spectrometer 7. Record the Intercept as well as the First, Second, and Third Coefficients. Also, look at the value for R squared. It should be very close to one. If it is not, you have probably assigned one of your wavelengths incorrectly. Keep these values at hand. Saving the New Calibration Coefficients: USB Mode Wavelength calibration coefficients unique to each NIR Spectrometer are programmed into an EEPROM memory chip on the NIR Spectrometer.
A: Calibrating the Wavelength of the NIR Spectrometer 12. Repeat Step 12 for all of the new values. 13. Click on the Save All Values button to save the information, and then Exit the USB Programmer software. The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the NIR Spectrometer.
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Appendix B Specifications How the Spectrometer Works Light passes through the NIR Spectrometer through a fixed optical path. The optical bench of the NIR Spectrometer has no moving parts that can wear or break; all the components are fixed in place at the time of manufacture. The following table describes each component in the NIR Spectrometer.
B: Specifications Name Description InGaAs Detector Each pixel on the detector responds to the wavelength of light that strikes it. Electronics bring the complete spectrum to the software. NIR Detector Specifications NIR512 NIR256-2.1 NIR256-2.
B: Specifications NIR Spectrometer – Slit Size vs. Resolution and Throughput The following chart illustrates the effect that varying slit sizes have on NIR512 Spectrometer resolution and throughput: 16 60 50 12 40 10 8 30 6 20 4 Relative Intensity Resolution (FWHM) 14 10 2 0 0 50 100 150 0 200 Slit Size (microns) NIR Spectrometer Specifications NIR512 NIR256-2.1 Dimensions NIR256-2.
B: Specifications Optical resolution (slit dependent) NIR512 NIR256-2.1 NIR256-2.5 4.2 – 14.0 nm FWHM With Grating N1:4.5 – 14.0 FWHM With Grating N2: 7.5 – 25.0 nm FWHM 7.5 – 25.0 nm FWNM Focal length f/4, 40mm Dynamic range 5000:1 for a single scan Fiber optic connector SMA 905 to single-strand optical fiber (0.
B: Specifications Grating Efficiency Curves 9-pin RS-232 Serial Port Pinout When facing the 9-pin RS-232 Serial Port connector on the NIR Spectrometer, the pins are numbered as: 9-pin RS-232 Serial Port Connector – Pin Definitions Pin # Description 2 RS232 Transmit 3 RS232 Receive 5 Ground 197-00000-512-02-0707 25
B: Specifications 15-pin Accessory Connector Pinout When facing the 9-pin RS-232 Serial Port connector on the NIR Spectrometer, the pins are numbered as: 15-pin Accessory Connector – Pin Definitions Pin # Description 3 +5V DC 8 External Software trigger pin 10 Ground LED Information The LED on the NIR Spectrometer has two states: Blinking and Solid. A blinking LED indicates that the thermo-electric cooler (TEC) is not active.
Appendix C NIR256-2.5µm Sensitivity When configuring a system for operation out to 2.5µm it is important that you consider the following details: • Short Integration Times: For the detector to be sensitive out to 2.5µm, the detector’s band gap energy must be small. Unfortunately, this raises the absolute level of the detectors dark signal. A typical dark signal at 34ms is shown below. While the detector functions at integration times greater than 50ms, this seems to be the realistic limit.
C: NIR256-2.5 µm Sensitivity The figure below shows the relative intensity of a NIR256-2.5 Spectrometer with a 25um slit directly coupled to an LS-1 Light Source with a 600um fiber.
Index A accessories, 2 Accessories, 7 Adobe Acrobat Reader, 3 C Calibrating, 15 calibration coefficients saving in Serial mode, 19 saving in USB mode, 18 CCD Detector, 22 configuring, 7 OOIBase32, 7 SpectraSuite, 7 D Data transfer rate, 24 Dimensions, 23 document audience, iii purpose, iii summary, iii Dynamic range, 24 I Installation, 5 Serial, 6 USB, 5 Installed Filter, 21 Installing, 5 interface options, 3 L LED, 26 M memory chip, 1 Mirror, 21 modular approach, 2 N NIR controls, 8 OOIBase32, 9 Spec
Index S shipment contents, 3 Slit, 21 SMA Connector, 21 software, 2 Software and Resources Library CD, 3 Specifications Detector, 22 Spectrometer, 23 spectral response, 24 SpectraSuite, 2, 7, 8 Spectroscopic Accessories, 7 U upgrades, iv USB-ADP-PC, 3 USB-CBL-1, 3 W Wavelength Calibration, 15 Wavelength Calibration Data File, 3 Wavelength Calibration Data Sheet, 3 Weight, 23 what's new, iii T troubleshooting Linux systems, 13 Mac systems, 13 serial port, 13 Windows systems, 11 Troubleshooting, 11 30 1
