HR2000 and HR2000CG-UV-NIR Series High-Resolution Fiber Optic Spectrometers HR2000 / HR2000CG-UV-NIR Installation and Operation Manual Document Number 194-00000-000-02-0208 Offices: Ocean Optics, Inc. 830 Douglas Ave., Dunedin, FL, USA 34698 Phone 727.733.2447 Fax 727.733.3962 8:30 a.m.-6 p.m. EST Ocean Optics B.V. (Europe) Nieuwgraaf 108 G, 6921 RK DUIVEN, The Netherlands Phone 31-(0)26-3190500 Fax 31-(0)26-3190505 E-mail: Info@OceanOptics.com Info@OceanOpticsBV.com Orders@OceanOptics.
Copyright © 2001-2008 Ocean Optics, Inc. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc. This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or otherwise circulated without the prior consent of Ocean Optics, Inc.
Table of Contents Chapter 1: HR2000 Introduction ...........................................................................................................1 Product Overview .................................................................................................................................................. 1 System Requirements ........................................................................................................................................................................
Chapter 5: Sample Experiments.........................................................................................................18 Preparing for Experiments ................................................................................................................................... 18 Absorbance Experiments..................................................................................................................................... 20 Transmission Experiments ..............................
HR2000 Introduction 1 HR2000 Introduction The following chapter contains introductory information about the HR2000 Spectrometer, shipment information, and spectrometer connectivity. Product Overview The HR2000 High-resolution Miniature Fiber Optic Spectrometer provides optical resolution as good as 0.065 nm (FWHM). The HR2000 is responsive from 200-1100 nm, but the specific range and resolution depends on your grating and entrance slit selections.
HR2000 Introduction EEPROM Utilization An EEPROM memory chip in each HR2000 contains wavelength calibration coefficients, linearity coefficients, and a serial number unique to each individual spectrometer. The OOIBase32 software application reads these values directly from the spectrometer, enabling the ability to “hot-swap” spectrometers between PCs without entering the spectrometer coefficients manually on each PC.
HR2000 Introduction Interface Options The HR2000 has both USB and serial port connectors (with the use of an adapter), enabling you to connect the spectrometer to a desktop or notebook PC via a USB port or to a desktop, notebook, or to a handheld PC via a serial port.
HR2000 Introduction Software and Technical Resources CD Each order ships with the Ocean Optics Software and Technical Resources CD. This disc contains software, operating instructions, and product information for all Ocean Optics software, spectrometers, and spectroscopic accessories. You will need Adobe Acrobat Reader version 6.0 or higher to view these files. Ocean Optics includes Adobe Acrobat Reader on the Software and Technical Resources CD.
HR2000 Specifications 2 HR2000 Specifications This chapter contains information on spectrometer operation, specifications, and system compatibility. It also includes accessory connector pinout diagrams and pin-specific information. How the HR2000 Works Below is a diagram of how light moves through the optical bench of an HR2000 Spectrometer. The optical bench has no moving parts that can wear or break; all the components are fixed in place at the time of manufacture.
HR2000 Specifications HR2000 Specifications Table Ocean Optics permanently secures all components in the HR2000 at the time of manufacture. Only Ocean Optics Technicians can replace interchangeable components, where noted. Item Name 1 SMA Connector 2 Slit* 3 Filter* 4 Collimating Mirror 5 Grating* 6 Focusing Mirror 7 L2 Detector Collection Lens* 8 CCD Detector (UV or VIS) Description The SMA Connector secures the input fiber to the spectrometer.
HR2000 Specifications HR2000 Specifications The following sections provide specification information for the CCD detector in the HR2000, as well as the HR2000 Spectrometer itself. CCD Detector Specifications Detector: No. of elements: Pixel size: Pixel well depth: Signal-to-noise ratio: A/D resolution: Dark noise: Corrected linearity: Sony ILX511 linear silicon CCD array 2048 pixels 14 µm x 200 µm 62,500 electrons 250:1 (at full signal) 12 bit 2.5 RMS counts >99.
HR2000 Specifications System Compatibility The following sections provide information on hardware and software requirements for the HR2000: Compatibility for Desktop or Notebook PCs To use the HR2000, you must have a PC that meets the following minimum requirements: • IBM-compatible PC with Pentium (or higher) processor • 32 MB RAM • OOIBase32 Spectrometer Operating Software • Windows 98/ME/2000/XP operating system (when connecting the HR2000 to a PC via USB port) or Any 32-bit version of Windows (w
HR2000 Specifications 20-Pin Accessory Connector Pinout Diagram When facing the 20-pin Accessory Connector on the HR2000, pin numbering is as follows: 20 18 16 14 12 10 8 6 4 2 19 17 15 13 11 9 7 5 3 1 Figure 2-3: 20-Pin Accessory Connector Pinout Diagram 20-Pin Accessory Connector – Pin Definitions The following table contains information regarding the function of each pin in the HR2000’s 20-Pin Accessory Connector: Pin # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Description
Installing the HR2000 3 Installing the HR2000 This chapter contains instructions in parallel for connecting the HR2000 via both USB and serial modes. Note: You must install the OOIBase32 software application prior to connecting the HR2000 Spectrometer to the PC. The OOIBase32 software installation installs the drivers required for HR2000 installation. If you do not install OOIBase32 first, the system will not properly recognize the HR2000.
Installing the HR2000 Configuring the HR2000 in OOIBase32 Once you install the HR2000, you must configure OOIBase32’s Configure Spectrometer options so that OOIBase32 recognizes the HR2000 Spectrometer. Note: Consult the OOIBase32 Spectrometer Operating Software Manual for detailed instructions on configuring the spectrometer in OOIBase32. You can find these instructions on the Software and Technical Resources CD or on the Ocean Optics web site at the following location: http://www.oceanoptics.
Installing the HR2000 Configure Hardware Screen - Continued USB Mode Serial Port Mode 1. Specify S2000/PC2000/USB2000/HR2000 in the Spectrometer Type drop-down menu. 2. Specify HR2000 in the A/D Converter Type drop-down menu. 3. Specify the serial number of the HR2000 under the USB Serial Number drop-down menu. Note: The system pre-fills this drop-down menu with the serial numbers of all discovered HR2000 Spectrometers. 4. Click the OK button to accept the selected options. 1.
Installing the HR2000 Spectrometer Configuration Screen The Spectrometer Configuration screen prompts you to configure specific channel-level spectrometer information, if necessary. Select Spectrometer | Configure | Wavelength Calibration tab from the menu and set system parameters. If you have connected your spectrometer to the PC’s USB port, OOIBase32 pre-fills the coefficients for the HR2000 from information on a memory chip in the spectrometer.
Troubleshooting 4 Troubleshooting The following sections contain information on troubleshooting issues you may encounter when using the HR2000 Spectrometer. Note: For issues encountered when using a handheld PC, consult the OOIPS2000 manual.
Troubleshooting 4. Click the OK button to continue. A warning box appears confirming the removal of the Unknown Device. Click the OK button to confirm the device removal. 5. Disconnect the HR2000 from your computer. 6. Locate the section in this chapter that is appropriate to your operating system and perform the steps in the “Remove Improperly Installed Files” section. Windows 98: Remove Improperly Installed Files: 1. Open Windows Explorer. 2. Navigate to the Windows | INF directory.
Troubleshooting Windows 2000/XP: Remove Improperly Installed Files: 1. Open Windows Explorer. 2. Navigate to the Windows | INF directory. If the INF directory is not visible, you will need to disable the “Hide System Files and Folders” option on in Windows Folder Options. Note: If the INF directory is not visible, you will need to disable the “Hide System Files and Folders” and “Hide File Extensions for Known File Types” options in Windows Folder Options.
Troubleshooting Troubleshooting the Serial Port Configuration Occasionally, you may encounter problems with the serial port connection and/or software. Perform the following steps to troubleshoot the serial port connection: 1. Cycle the power on the HR2000 and restart the OOIBase32 software. This ensures that the software and the HR2000 are synchronized. 2. Determine the serial port (COM port) number: Operating System Windows 95/98/ME Windows 2000/XP Windows NT Instructions 1.
Sample Experiments 5 Sample Experiments The following sections contain information on conducting sample experiments using the HR2000 and OOIBase32. For information on experiments with OOIPS2000, consult the OOIPS2000 Operating Instructions. Preparing for Experiments Follow the steps below to configure the HR2000 and OOIBase32 for experiments: 1. Double-check that you have correctly installed the HR2000, installed OOIBase32, and configured the light source and other sampling optics. 2.
Sample Experiments Application Tips If the signal you collect is saturating the spectrometer (intensity greater than 4000 counts), you can decrease the light level on scale in scope mode by: • Decreasing the integration time • Attenuating the light going into the spectrometer • Using a smaller diameter fiber • Using a neutral density filter with the correct optical density If the signal you collect has too little light, you can increase the light level on scale in scope mode by: • Increasing the in
Sample Experiments Absorbance Experiments Absorbance spectra are a measure of how much light a sample absorbs. For most samples, absorbance is linearly related to the concentration of the substance. OOIBase32 calculates absorbance (Aλ) using the following equation… Aλ = - log10 Sλ - Dλ ( R -D ) λ λ …where Sλ is the sample intensity at wavelength λ, Dλ is the dark intensity at wavelength λ, Rλ is the reference intensity at wavelength λ. Figure 6-1: Typical absorbance setup.
Sample Experiments 3. Place a sample of the solvent into a cuvette and take a reference spectrum. You must take a reference spectrum before measuring absorbance. Note: Do not put the sample itself in the path when taking a reference spectrum, only the solvent. Click the Store Reference spectrum icon on the toolbar or select Spectrum | Store Reference from the menu bar to store the reference. This command merely stores a reference spectrum in memory.
Sample Experiments Transmission Experiments Transmission is the percentage of energy passing through a sample relative to the amount that passes through the reference. Transmission Mode also displays the portion of light reflected from a sample, since transmission and reflection measurements use the same mathematical calculations. The transmission is expressed as a percentage (%Tλ) relative to a standard substance (such as air).
Sample Experiments 6. Place a sample of the solvent into a cuvette and take a reference spectrum. You must take a reference spectrum before measuring transmission. Note: Do not put the sample itself in the path when taking a reference spectrum, only the solvent. Click the Store Reference spectrum icon on the toolbar or select Spectrum | Store Reference from the menu bar to store the reference. This command merely stores a reference spectrum in memory.
Sample Experiments Reflection Experiments Reflection is the return of radiation by a surface, without a change in wavelength. Reflection can be: • Specular (the angle of incidence is equal to the angle of reflection) • Diffuse (the angle of incidence is not equal to the angle of reflection) Every surface returns both specular and diffuse reflections. Some surfaces may return mostly specular reflection, while others may return mostly diffuse reflection.
Sample Experiments Perform the following steps to take reflection measurements using OOIBase32: 1. Place OOIBase32 is in scope mode by clicking the Scope Mode icon on the toolbar, or by selecting Spectrum | Scope Mode from the menu bar. 2. Ensure that the entire signal is on scale. The intensity of the reference signal should peak at about 3500 counts. 3. Take a reference spectrum with the WS-1 Diffuse Reflectance Standard or the STAN-SSH Highreflectivity Reference Standard.
Sample Experiments Relative Irradiance Experiments Irradiance is the amount of energy at each wavelength emitted from a radiant sample. In relative terms, it is a comparison of the fraction of energy the sample emits and the energy the sampling system collects from a lamp with a blackbody energy distribution (normalized to 1 at the energy maximum).
Sample Experiments Perform the following steps to take a relative irradiance measurement using OOIBase32: 1. Place OOIBase32 is in scope mode by clicking the Scope Mode icon on the toolbar, or by selecting Spectrum | Scope Mode from the menu bar. 2. Ensure that the entire signal is on scale. The intensity of the reference signal should peak at about 3500 counts. Note: The light source must be a blackbody of known color temperature. 3.
Sample Experiments Time Acquisition Experiments OOIBase32 allows you to perform time acquisition experiments. Time acquisition experiments track processes, perform kinetic analyses, and monitor spectral events all as a function of time. You can collect, as a function of time, spectral data from up to six single wavelengths (designated as Channels A through F) and up to two mathematical combinations of these wavelengths (designated as Combinations 1 and 2).
Sample Experiments 3. Configure a time acquisition process for a combination of two time channels (if desired) by selecting Combination 1. Perform the steps below to configure a combination: a. Select Enabled to set the time acquisition calculation for the wavelength. b. Enable Plotted to see a real-time graph of the acquired data in a spectral window. c. Specify Time Channel A through F for the First Channel. d. Select the mathematical operation to produce the data for Combination 1. e.
Sample Experiments 6. Enable Save Every Acquisition to store data for every spectral acquisition during a time acquisition process (optional). Note: OOIBase32 has options to either store data for each acquisition, or to collect data only after a specified delay. Several factors affect the minimum time acquisition frequency, including integration time, number of spectrometer channels, samples averaged, and computer speed.
Appendix A Appendix A: Calibrating the Wavelength of the HR2000 This Appendix describes how to calibrate the wavelength of your spectrometer. Although each spectrometer is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the HR2000.
Appendix A Calibrating the Wavelength of the Spectrometer Perform the steps below to calibrate the wavelength of the spectrometer: 1. Place OOIBase32 into Scope Mode and take a spectrum of your light source. Adjust the integration time (or the A/D conversion frequency) until there are several peaks on the screen that are not off-scale. 2. Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum intensity. 3.
Appendix A 5. Use the spreadsheet or calculator to calculate the wavelength calibration coefficients. In the spreadsheet program, find the functions to perform linear regressions. • If using Quattro Pro, look under Tools | Advanced Math • If using Excel, look under Analysis ToolPak 6. Select the true wavelength as the dependent variable (Y). Select the pixel number, pixel number squared, and the pixel number cubed as the independent variables (X).
Appendix A Saving the New Calibration Coefficients: USB Mode Ocean Optics programs wavelength calibration coefficients unique to each HR2000 onto an EEPROM memory chip in the HR2000. You can overwrite old calibration coefficients on the EEPROM if you are using the HR2000 via the USB port. If you are using the HR2000 via the serial port, consult the Saving the New Calibration Coefficients: Serial Mode section later in this Appendix. To Save Wavelength Calibration Coefficients Using the USB Mode 1.
Appendix A Saving the New Calibration Coefficients: Serial Mode If you are connecting the HR2000 Spectrometer to the serial port of the PC, you need to save the new wavelength calibration coefficients to the .SPEC file that OOIBase32 accesses when opened. Note: You cannot save the calibration coefficients to the EEPROM memory chip on the HR2000 when using the serial mode. To Save Wavelength Calibration Coefficients Using the Serial Mode 1. Open the OOIBase32 application. 2.
Appendix B Appendix B: HR2000CG-UV-NIR Spectrometer The HR2000CG-UV-NIR Composite Grating Spectrometer has a new proprietary grating and order-sorting filter to provide a 200-1100 nm wavelength range with 1.0 nm optical resolution in one spectrometer. The HR2000-CG-UV-NIR is functionally similar to the standard HR2000 Spectrometer. Follow the instructions in Chapter 3 to configure the HR2000CG-UV-NIR.
Index A Accessories, 2, 13 Accessory Connector, 8 L L2 Detector Collection Lens, 6 Lens, 6 Diagram, 9 Pin Definitions, 9 Pinout, 8 M memory chip, 2 Mirror, 6 Adobe Acrobat Reader, 4 C Calibrating, 31 CCD, 7 CCD Detector, 6 Compatibility, 8 Component Table, 5 Configure Hardware, 11 Configuring, 11 O OOIBase32, 11 Options Interface, 3 Overview, 1 Sampling System, 2 P D Default Spectrometer Configuration File, 11 Detector, 7 Detector Collection Lens, 6 E EEPROM, 2 Experiments, 18 Packing List, 3 Pas
W Wavelength Calibration, 31 Wavelength Calibration Data File, 3 Wavelength Calibration Data Sheet, 3 HR2000 High-Resolution Fiber Optic Spectrometer 38
