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ManualsBrandsOcean Optics ManualsEquipmentExternal Triggering Options

200-00000-000-01-201401 1

External Triggering Options Instructions

Overview

Ocean Optics USB2000, USB2000+, USB4000, HR2000, HR2000+, HR4000, Jaz, Maya, QE65000, NIR

Series, NIRQuest Series, S2000, and S1024DW Spectrometers provide several methods of acquiring data

(see table below). In the Normal mode, the spectrometer is “free running.” That is, the spectrometer is

continuously scanning, acquiring, and transferring data to your computer, according to parameters set in

the software. In this mode, however, there is no way to synchronize the scanning, acquisition, and transfer

of data with an external event. However, trigger pulses for synchronizing an external event with the

spectrometer are available.

To synchronize data acquisition with external events, other modes of acquiring data are available. Each

mode involves connecting an external triggering device to the spectrometer and then applying an external

trigger to the spectrometer before the software receives the data. The length of the integration time and

the source for the integration clock depend upon the mode chosen. All other acquisition parameters are set

in the software.

For spectrometers with FPGA firmware versions of 3.0 or higher, and for spectrometers not covered by

this document, see

New External Triggering Options Instructions for Spectrometers with Firmware

Version 3.0 and Above. Also see the HR4000 and USB4000 Shutter Mode Performance in Hardware

Trigger Mode for more information on external triggering with these spectrometers.

Triggering Mode Description Applicable Spectrometer(s)

Normal Spectrometer acquires spectra continuously. All

External

Software

Integration time is set in the software. Software

receives a trigger event and transmits spectra obtained

in the data acquisition cycle in which the trigger

occurred.

All

External

Hardware

Integration time set via chip in spectrometer (USB

Series/Jaz/HR Series/S2000 D Series) or jumpers

(S2000 series). Spectrometer waits for a sharp rise in

voltage on the trigger input pin, and then acquires

spectra until the voltage is removed.

USB2000/USB4000/Jaz/

HR2000/HR2000+/HR4000/

S2000/S1024DW

External

Synchronization

Spectrometer acquires data from an external trigger

event (such as a push button) until the next time the

trigger is activated, at which time the spectrometer

ceases spectral acquisition and begins a new

acquisition. Integration time cannot be set, since the

trigger can fire at random intervals.

S2000/S1024DW

Summary of content (14 pages)

PAGE 1
External Triggering Options Instructions Overview Ocean Optics USB2000, USB2000+, USB4000, HR2000, HR2000+, HR4000, Jaz, Maya, QE65000, NIR Series, NIRQuest Series, S2000, and S1024DW Spectrometers provide several methods of acquiring data (see table below). In the Normal mode, the spectrometer is “free running.” That is, the spectrometer is continuously scanning, acquiring, and transferring data to your computer, according to parameters set in the software.
PAGE 2
External Triggering Options Instructions Triggering Mode Description Applicable Spectrometer(s) Quasi-Real Time Acquisition Mode The integration clock is set to 4ms, which is the time required to completely read out all the pixels. When the software (user) requests a spectrum, the integration clock waits for the current period to expire, changes to the desired integration time set via software commands, and then returns to 4ms.
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External Triggering Instructions Trigger Input Pin Information for Ocean Optics Spectrometers Trigger Input Pin Trigger Mode S2000 S1024DW QE65000 USB2000 USB2000+ USB4000 HR2000 Jaz HR4000 HR2000+ NIR Series NIRQuest Series Description External Software 8 10 7 10 10 Asynchronous Mode Integration time specified in software External Synchronization 5 Not Available Not Available Not Available Not Available Synchronous Mode - User provides integration clock External Hardware 4 Not Avail
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External Triggering Options Instructions Level Triggering and Edge Triggering In External Triggering, there are two modes of triggering available. The trigger mode in use determines the kind of triggering that the system will use. Level Triggering Level triggering mode is only available when using External Software Triggering mode. In this mode, the software monitors the A/D converter for an increase in the voltage level applied to the trigger pin.
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External Triggering Instructions Integration Times Available with External Triggering The following table illustrates the integration times available with External Triggering modes: Spectrometer External Software External Hardware External Synchronization USB2000/HR2000 Set in Software 50 msec Cannot Manually Set USB4000/USB2000+/Jaz/HR2000+/HR4000 Set in Software Set in Software Input by software NIR Series Set in Software Not Available Not Available NIRQuest Series Set in Software Not A
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External Triggering Options Instructions Product Supported Not Supported OOISensors External Software External Synchronization and External Hardware OOIIrrad External Software and External Hardware External Synchronization Palm Spec External Software External Sync and External Hardware Configuring External Triggering External Software Trigger Mode In this level-triggered mode, the spectrometer is “free running,” just as it is in the Normal mode.
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External Triggering Instructions Using the External Software Trigger Mode ► Procedure 1. Connect the line to the appropriate pin on the spectrometer: USB2000, USB2000+, USB4000, Jaz, and HR2000 Spectrometers Supply a line from Pin 7 of the Multi-pin Connector on the spectrometer to your triggering device. HR2000+, HR4000 and QE65000 Spectrometers Supply a line from Pin 10 of the Multi-pin Connector on the spectrometer to your triggering device.
PAGE 8
External Triggering Options Instructions Once you select an external trigger mode, your computer will appear unresponsive. This is normal, as the computer is waiting for a trigger. You must apply one more trigger to the spectrometer after selecting a new trigger mode.
PAGE 9
External Triggering Instructions Using the External Synchronization Trigger Mode ► Procedure 1. Supply a line from Pin 5 of the J2 Accessory Connector to the triggering device. (See figure in Pinout Diagrams for Ocean Optics Spectrometers on page 2 for pin location.) 2. Connect Pin 10 of the J2 Accessory Connector (ground) to the ground of the triggering device. 3.
PAGE 10
External Triggering Options Instructions External Hardware Triggering – Trigger Timing When to Use External Hardware Triggering Mode Use the External Hardware Triggering mode if you: • • • Are using a pulsed excitation source or light source in your experiment (such as a laser or flash lamp) Are doing LIF (fluorescence with pulsed excitation) or phosphorescence experiments Need to synchronize an acquisition with an external event Using External Hardware Triggering Mode Follow the steps below to enable E
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External Triggering Instructions If you enable this function, you will be presented with a file save dialog box with each trigger. To avoid manually naming a file for each trigger, you can enable the Autoincrement Filenames function by selecting File | Autoincrement Filenames | Enabled from the menu. Choose a base name and starting index for the autoincremented files. Note Once you select the External Hardware Trigger mode, your system may appear unresponsive.
PAGE 12
External Triggering Options Instructions When to Use External Hardware Trigger Mode Use the External Hardware Trigger mode if you: • • • • Are using a pulsed excitation or light source in your experiment Are doing LIF (fluorescence with pulsed excitation) or phosphorescence experiments Are able to jumper the pins on Jumper Block 4 of the spectrometer’s electronic board Need to synchronize an acquisition with an external event Using the External Hardware Triggering Mode ► Procedure Supply a line from Pin
PAGE 13
External Triggering Instructions S1024DW Miniature Fiber Optic Spectrometer Integration Time SAD500 (500 kHz Master Clock Freq) Jumper Block 4 (JP4) Integration Time Equation Pins 1-2 Pins 3-4 5 ms 1000/Fm open open 60 ms 12000/Fm open shorted 300 ms 60000/ Fm shorted open 655 ms 131070/ Fm shorted shorted Fm = the master clock frequency in Hz (i.e., 200 kHz for the SAD500); shorted = connected; open = disconnected ► Procedure 1.
PAGE 14
External Triggering Options Instructions 7. Enable the Automatically save file on trigger box if you wish to save the data to a file after each trigger. If you enable this function, OOIBase32 displays a file save dialog box after each trigger. To avoid manually naming a file for each trigger, you can enable the Autoincrement Filenames function from the File | Autoincrement Filenames | Enabled option in the menu bar. Note Once you select an external trigger mode, your computer will appear unresponsive.