Temperature-regulated Cuvette Holder CUV-TLC-50F Installation and Operation Manual Document Number 000-30000-000-02-0205 Offices: Ocean Optics, Inc. 830 Douglas Ave., Dunedin, FL, USA 34698 Phone (727) 733-2447 Fax (727) 733-3962 8:30 a.m.–6 p.m. EST Ocean Optics B.V. (Europe) Nieuwgraaf 108 G, 6921 RK DUIVEN, The Netherlands Phone 31-(0)26-3190500 Fax 31-(0)26-3190505 E-mail: Info@OceanOptics.com Info@OceanOpticsBV.com Orders@OceanOptics.com TechSupport@OceanOptics.
Copyright © 2001-2005 Ocean Optics, Inc. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc. This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or otherwise circulated without the prior consent of Ocean Optics, Inc.
Table of Contents About This Manual .......................................................................................................... iii Document Purpose and Intended Audience ............................................................................. iii What’s New in this Document ................................................................................................... iii Document Summary................................................................................................
Table of Contents Chapter 3: Specifications ...................................................................13 Specifications Table ........................................................................................................ 13 Temperature .................................................................................................................... 13 Variable Speed Magnetic Stirring .................................................................................... 14 Dry Gas Purge..
About This Manual Document Purpose and Intended Audience This document provides you with an installation section to get your system up and running. In addition to the Maxwell system installation and operation instructions, this manual also includes information for locating the OOIBase32 installation instructions (see Product-Related Documentation below).
About This Manual Product-Related Documentation You can access documentation for Ocean Optics products by visiting our website at http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate document from the available drop-down lists. Or, use the Search by Model Number field at the bottom of the web page. You can also access operating instructions for Ocean Optics products on the Software and Technical Resources CD included with the system.
Chapter 1 Introduction Overview The CUV-TLC-50F Temperature-regulated Cuvette Holder is a high-quality, versatile sample chamber with a Peltier temperature controller calibrated against a NIST-traceable thermometer. The device controls the temperature of the holder from -40 °C to 85 °C and maintains a constant temperature within ±0.02 °C. The CUV-TLC-50F includes the cuvette holder and the external temperature controller box.
1: Introduction Features Features of the CUV-TLC-50F include the following: • • Magnetic stirring (in the CUV-TLC-50F). A stir bar is included. Dry gas purge: Limits condensation and excludes oxygen from around the cuvette. Tubing for water and gas connections is included. • Optical slits: Control the illuminated volume. Several removable optical slits are included.
1: Introduction Components CUV-TLC-50F Components Optional Equipment The following optional components and packages are also offered for specific applications. Contact Ocean Optics for additional information.
1: Introduction CUV-TLC-IL Imaging Lens The CUV-TLC-IL is an AR-coated fused silica imaging lens doublet with SMA 905 connector and fiber optic steering plate. The CUV-TLC-IL imaging lens doublet is identical to the CUV-TLC-CL, except that an additional lens is snapped into place to shorten the focal length. The imaging lens images the end of the optical fiber into the cuvette, or focuses an image of the center of the cuvette onto a fiber.
1: Introduction Additional Accessories The following additional accessories are available from Ocean Optics for your CUV-TLC-50F: • CUV-TLC-ADP PC adapter package for remotely operating the cuvette holder. The serial interface is used for external computer control of the temperature controller.
1: Introduction Packages for Specific Applications Application Item Code Description UV/VIS spectroscopy CUV-TLC-ABSKIT CUV-TLC 50F cell holder, two CUVTLC-CL collimating lenses (with SMA fiber optic connectors, lenses and steering plates), CUV-TLC-ADP serial interface and CUV-TLC-BATH water pump with bucket.
Chapter 2 Operation Optics The CUV-TLC-50F provides ports for viewing or illuminating 1-cm square cuvettes from four directions. Lenses are available to purchase separately to allow you to choose the best lenses for your application. For absorbance and transmission measurements, position two CUV-TLC-CL Collimating Lenses at 180°.
2: Operation Typical Configuration for Fluorescence Measurements To measure fluorescence, Imaging Lens systems are placed at 90 degrees to each other. The first lens system focuses the light as a small image inside the cuvette Emitted light from this small illuminated volume is then focused at a right angle onto the second fiber. Mirrors can be mounted opposite each lens system, nearly quadrupling measured light intensities.
2: Operation Caution Be sure to first loosen the fiberoptic SMA 905 connection, so as not to apply torsion to the fiber. This adjustment varies the position of focus in the sample when desired for specific experiments, or to compensate for lens aberrations to maximize optical throughput. For normal operation the distance between the knurled ring on the horizontal fiber position knob should be set 1/8 inch from the knurled ring on the first lens holder (see the following figure).
2: Operation Note The CUV-TLC-50F is intended to hold a cuvette with a standard wall thickness of 1.25 mm and total width of 12.5 mm. Cuvettes with unusually thick walls will not properly fit and may damage the holder. Also, unusually short cuvettes will be difficult to remove after being pushed all of the way to the bottom of the holder. 3. Place the cuvette and sample in the sample holder. 4.
2: Operation Temperature Equilibration The serial control option was used to automatically cycle the CUV-TLC-50F through a series of temperatures. The blue line shows the resulting temperature of the sample holder next to one of the optical ports. The red line shows the temperature measured inside a cuvette. The cuvette, containing ethylene glycol and water, was stirred using the magnetic stirring feature of the CUV-TLC-50F.
2: Operation Temperature Ramping Each of the sample holders from Quantum Northwest has a function for temperature ramping programmed into its microcontroller. The user first allows equilibration at a particular temperature. To initiate the ramp, the user then specifies a final temperature and increments for temperature and time. The serial control option, allowing external control from a computer, is required for temperature ramping.
Chapter 3 Specifications Specifications Table Specification Value Temperature -40 to 85 °C Precision ±0.02 °C Reproducibility ±0.05 °C Maximum Luminated Area (h x w) 12 x 10 mm Height of Optical Center Above Table 70 mm Optical Center Above Bottom of Cuvette 8.5 mm Magnetic Stirring yes Dry Gas Purge yes Temperature Thermoelectric temperature control maintains the sample to a precision of ±0.02 °C within a range of -40.00 °C to +85.00 °C.
3: Specifications Variable Speed Magnetic Stirring Variable speed magnetic stirring is provided for experiments in which it is important to rapidly remove photoproducts from the illuminated volume. A stir bar is included. Dry Gas Purge A dry gas purge is provided. The gas travels through the base of the cuvette holder for temperature equilibration and then blows onto each of the four faces of the cuvette. The gas purge minimizes condensation on optical surfaces.
3: Specifications Mechanical Diagram 000-30000-000-02-0205 15
3: Specifications 16 000-30000-000-02-0205
Appendix A Serial Control for the CUV-TLC-50F Overview Serial Control permits the remote temperature control via a serial interface on a computer using a Windows operating system. The option consists of a cable that connects the Temperature Controller with the computer, and software. The software includes QNW_SC.exe, a sample control program that enables control, and recovers and plots temperature data.
A: Serial Control for the CUV-TLC-50F Serial Control from Other Programs It may be useful to have temperature control for the CUV-TLC-50F system from other programs (for example data acquisition programs). The installation CD includes code for a simple C++ program that illustrates how to use QnwSerial.DLL for serial communications with the TLC 50™ hardware. QnwSerial.dll provides functions that simplify opening and closing serial ports, and sending and receiving data over a serial connection.
A: Serial Control for the CUV-TLC-50F The list file also may contain a list of command lines which QnwExample loads and inserts into a program drop down list to give the user access to the commands. To use the program, the user either chooses one of the commands from the drop down list (and possibly edits it) or enters a command directly into the edit control provided. That command is sent out the serial port when the user presses the “Send Command” button.
A: Serial Control for the CUV-TLC-50F Table 1: Serial Commands (Cont’d) Command Response [F1 IS ?] Query: What is the current instrument status? [F1 IS 0+-S] Reply: no unreported error (0 or 1) temperature control is on (+ or -) temperature is stable (S or C) stirrer is off (+ or -) [F1 IS +5] Automatically report instrument status every 5 seconds [F1 IS +] Automatically report instrument status whenever it changes (e.g.
Index A absorbance measurements, 7 accessories, 5 E error conditions, 12 example programs, 18 B bath, 5 C collimating lens, 3, 9 components, 3 configuration absorbance measurements, 7 fluorescence measurements, 8 CUV-TLC-ABSKIT, 6 CUV-TLC-ADP, 5 CUV-TLC-BATH, 5 CUV-TLC-CL, 3 CUV-TLC-FH, 4 CUV-TLC-FLKIT, 6 CUV-TLC-IL, 4 CUV-TLC-MP, 4 CUV-TLC-MPKIT, 6 CUV-TLC-SP, 5 D document audience, iii purpose, iii summary, iii dry gas purge, 14 000-30000-000-02-0205 F features, 2 filter holder assembly, 4 fluoresce
Index P PC adapter, 5 performance, 10 product-related documentation, iv purge dry gas, 14 T temperature, 13 temperature equilibration, 10 temperature ramping, 11 U R ramping commands, 20 ramping control, 19 upgrades, iv UV/VIS spectroscopy, 6 W S what's new, iii serial commands, 19 serial control, 17 from other programs, 18 setup and software installation, 17 uninstalling software, 17 serial interface, 14 specifications, 13 spherical mirror plug, 4 steering plate, 5 system operation, 9 system setup,
