Manual

Overview
The Legionella Systems Test Kit is for detecting Legionella in a water sample -- it is ideal for on-line and a variety
of industrial applications.
The test operates via a Lateral Flow Immuno-Chromatographic Assay (LFICA).
Each Systems Test Kit contains the following:
 •5Individuallyfoil-wrappedtests--eachcontainsanexactvolumepipette
 •5HollowFiberFilters
 •5SyringeswithRecoveryBuffer
 •1SampleCollectionBottle
 •1Samplepointpipeadapter
 •Instructionsforuse
This test is intended for analysis of water samples only. This product is not intended for clinical or medical
diagnostic use.
Theproductisintendedtobeusedaspartofanoverallwatertreatment,management,andriskreductionap-
proachandshouldNOTbeusedasthesolemethodforassessingrisksassociatedwithLegionellabacteria.
Test Operating Limits
Thetesthasbeenevaluatedforoperationbetween10-40ºC(50-104ºF).
Thetesthasbeenvalidatedforsamplesthatlterinlessthan10minutes.Samplesrequiringmorethan10min-
utestoltermaygiveerroneousresults.Samplesthatrequirelongtimestosamplemaybeindicativeofpoor
systemmaintenance.Awiderangeofnon-oxidizingbiocidesandbiodispersantshavebeencheckedforcross
reaction and inference with the test. This test should not be used on systems treated with biguanide or
THPS based biocides!
PerformanceofthetestwithsamplesotherthanthosetakenfromCoolingTowers,DomesticHot&Coldwater
systemsandWhirlpoolspashasnotbeenestablished.
Disposal
Thetest,lter,syringeandcapscannotbereusedorrecycled.Thepackagingmaterialsandthisinstruction
manualcanberecycled.
Storage
Thetestisintendedforstorageatroomtemperature.DoNotFreeze.Whenstoredcorrectly,thetestwill
continuetooperatewithindesignspecications,untilthespeciedexpirationdate.Do not use the test after
the stated expiration date specied on the packaging, Do not use any test where the foil packaging
is damaged.
Technical Information
Limit of Detection
Laboratoryanalysishasdemonstratedthat98-100%(91%Cl)oftestsarepositiveforcleanwatersamples
containing100CFU/LiterLegionella pneumophilaserogroup1.Thetheoreticalmathematicallimitofdetec-
tionofthetestisequivalent100CFU/Lwhena250mlsampleisltered.Ifsmallervolumesareprocessed,the
detectionlimitwillbealteredaccordingly:
Suspendedsolidcontentinthewatersamplesimpactsltrationandtestperformance,includinganalyticalsen-
sitivity.Acutualresultswillvary.Watersampleswithhighlevelsofsuspenedsolidsmayblockltrationentirely.
Legionella pneumophilaserogroup1bacteriarecoveryfromwatersamplescanrangefrom<10to100%,
dependingonwatersamplecomposition.Thisissimilartoltrationconcentrationtechniquestusedinother
microbiologicalanalysismethods.
limit of detection =
100CFU
Sample Volume (L)