Manual
Example of a new test Example of a positive test
Example of a negative test
Read the Test Strip
Leavetheteststripsittingonaatsurfaceduringincubation.Foroptimumresultsthetestresultsshouldbe
performed at room temperature. After waiting 25 minutes, examine the test strip in good lighting. If the
testisnotreadwithin60minutesofaddingthewatersample,itshouldbediscardedandanewtestshouldbe
run. The test should show one of the following results on the large result window on the test strip:
Negative Result:One(1)REDlineacrosstheresultwindowattheendfurthestfromthesamplewindow.
Positive Result:Two(2)REDlinesacrosstheresultwindow.Theredlineclosesttothesamplewindowmay
be very faint (pale pink). Any distinct line, no matter how faint, should be counted as positive.
Invalid Result: If the test does not show any red lines; or if it only shows a line at the end closest to the sample
window;orifthelinefurthestfromthesamplewindowisveryfaint,thenthetestisinvalid.Repeatthetestand
notify your supplier for technical support.
Interpreting the Results
Positive Results:
A positive test result indicates that Legionella pneumophila serogroup 1 bacteria were present in the sample
above the detection limit.
The test does not differentiate between viable (living) and non-viable (dead) organisms. The test will detect
viablebutnon-cultruablebacteriawhicharenotdetectablebytraditionallaboratorytechniques.Apositive
result does not necessarily mean that viable bacteria are present.
When a positive result is observed, seek advice from your risk management plan, or water treatment specialist.
Negative Results:
A negative test result indicates that Legionella pneumophila serogroup 1 bacteria were not detected or the
number of bacteria in the sample were below the detection limit.
A negative result does not necessarily mean that Legionella bacteria are absent.
A negative result does not mean that the system is completely free from risks associated with Legionella bacte-
ria. The test only detects Legionella pneumophila serogroup 1. The test does not detect the presence of other
Legionella species or serogroups.
Test Procedure, continued.
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