Manual

Resuspend the Bacteria
Disconnect the lter from the syringe. Disgard the large syringe. The hollow ber lter has a white cap on the
oppositeendofthesideusedforattachingtothesyringe.Removethiscapandswapends,sothatthecapis
now screwed into the place where you attached the sampling syringe. by twisting the lock ring and pulling the
lter from the tting.
Now, take a small syringe of recovery buffer, remove the red cap and attach it to the open end of the hollow
ber lter.
 •Pullthesyringebacktothe0.5mlmarktosuspendtherecoverybuffer,thenpushthesyringeall
 thewaytothe0mlmark.
 •Repeatthisprocesstwo(2)additionaltimes.
 •Drawthesyringebacktocollect0.1mlofsample,thendisconnectfromthelter.Thesyringe
now conains the recovered bacteria ready for testing.
Add Sample to the Test Strip
Removethetestfromitsfoilwrapping,andplacethetestonaatsurface.
The foil wrapping should not be opened until immediately prior to running the test. If the foil is opened and
thenthetestisnotperformedwithin60minutes,discardthetest.
Beforeuse,thetestshouldhave2palebluelinesacrosstheresultwindow.Ifthesearenotpresent,notifyyour
supplier to replace the test kit. Take the pipette from the foil wrapping.
Placethesyringeoverthesmallsamplewindowattheendoftheteststrip,andpresstheplungersothat0.1ml
of sample is dispensed onto the test strip. Record the time.
Take the Sample
Beforetakingthesample,ushthesampleportfor15seconds.Collectawatersampleofatleast250mlina
clean container.
Fromthekit,takea60mlsyringeanddrawup50-60mlofthesample.RemovetheHollowFiberFilterfromthe
packaging and attach it to the luerlock edge of the syringe. Filter the sample over a sink or other waste water
outlet.Repeatthisprocessuntilalloftheoriginal250mlsamplehasbeenltered.Thisshouldtakenolonger
than10minutes.
Test Procedure - Filtered Test for Water Samples
For how to read and interpret the test results, see page 6.
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