Enterprise Test Kit Legionella pneumophila serogroup 1 in Water & Biofilms Instruction Manual Part Number: L56B006501 www.orbeco.com sales@novatech-usa.com www.novatech-usa.
Technical Information Overview This test kit is used to detect the presence of Legionella pneumophila serogroup 1 bacteria in hot and cold water systems, cooling water, whirlpool spas and in biofilm. The test operates via a Lateral Flow Immuno-Chromatographic Assay (LFICA).
Specificity The test has been shown to be non-reactive with the following bacteria ( at 1 x 108 organisms per sample): Acinetobacter calcoaceticus, Aeromonas hydrophila subsp.
Test Procedure - Swab Test for Biofilms Collect Biofilm Sample Identify the water system to be sampled and an appropriate location from which to obtain a biofilm sample. Large systems may need to be sampled and tested at multiple locations. The recommended minimum area to swab is 10 cm2. If the surface to be sampled is dry, then pre-moisten the swab in recovery buffer, using the resuspension tube. Wipe the swab across the area to be tested.
Test Procedure - Filtered Test for Water Samples Take the Sample Before taking the sample, flush the sample port for 15 seconds. Collect a water sample of at least 250 ml in a clean container. From the kit, take a 60 ml syringe and draw up 50-60 ml of the sample. Remove the Hollow Fiber Filter from the packaging and attach it to the luerlock edge of the syringe. Filter the sample over a sink or other waste water outlet. Repeat this process until all of the original 250 ml sample has been filtered.
Test Procedure, continued. Read the Test Strip Leave the test strip sitting on a flat surface during incubation. For optimum results the test results should be performed at room temperature. After waiting 25 minutes, examine the test strip in good lighting. If the test is not read within 60 minutes of adding the water sample, it should be discarded and a new test should be run.
Orbeco-Hellige, Inc. 6456 Parkland Drive Sarasota, FL 34243 Phone: 941.756.6410 Fax: 941.727.9654 Email: service@orbeco.com Web: www.orbeco.com HYDROSENSE is a trademark of Albagaia Limited, used worldwide and registered in various territories.
