User Manual

Brine Shrimp Experiment
Equipment needed:
1. Hatchery or any household container
2. Sea salt (not included)
3. Brine Shrimp egg specimen vial
4. Yeast specimen vial
Incubation of Brine Eggs
The first step is to create a salt solution that corresponds to the living conditions of
the shrimp. Start with 2 tablespoons (1 fluid ounce) of water – bottled or filtered
water is recommended – in a small container. If filtered water is not available, tap
water can be used, but it should be left to sit for approximately 24 hours (this is to
dissipate any chemicals in the water). Add ½ teaspoon of sea salt to the container
and stir until all of the salt is dissolved. The water should be room temperature
(70-75°F), not hot or cold. Add a pinch of the brine eggs. Place the container in a
bright location, but not in direct sunlight. One option is to place the container
directly under a desk light or somewhere similar. You can also put some salt
solution along with a few eggs into each of the three compartments of the hatchery.
Leave the lid slightly off or completely off for the brine shrimp eggs to receive
oxygen. The ideal temperature should be around 70-75°F. At this temperature the
shrimp will hatch in about 2-3 days. If the water in the glass evaporates, repeat the
process of incubation without adding brine shrimp eggs and pour that new salt
solution into your existing experiment. Now sit back and enjoy the results of
your experiment, scientist!
12
The My First Lab
TM
Mini Duo-Scope has the capability to illuminate the
specimen from the top or the bottom. Notice the power switch located at the side
of the scope. This is a 3 way switch to operate either the bottom light, (set to the
single line), top light, (set to the double line), or set to the off position. (Circle in
between the two lines).
Operation
Compound Microscope Use (for slides)
Begin with the stage platform at its lowest position. To observe specimens, check
the objective lens, and if necessary, rotate the nosepiece so that the 4X objective
lens is in position for viewing. The lens will “click” when in place. Always start
viewing any specimen with the 4X objective and increase accordingly.
Begin by selecting a prepared slide and placing it carefully on the stage (labeled
side up). Lift gently on the front of the clips to lift them enough to slip the slide
under and hold it in place. The main part of the specimen should be centered over
the opening in the stage.
Turn on the lower illuminator (single line) to shine the light up through the stage
and through the tissue specimen on the slide.
5
Rotate the nosepiece
4X Objective
Rotate the nosepiece to 4X objective for viewing
and your image is magnified 40X