PH01 Perfusion Cannula Manual
Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH. While every precaution has been taken in the preparation of this document, the publisher and the author assume no responsibility for errors or omissions, or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it.
PH01 Manual Table of Contents 1.1 1.2 1.3 2.1 2.2 2.3 2.4 2.5 2.6 3.
PH01 Manual 1 Important Information and Instructions 1.
PH01 Manual 1.3 Important Safety Advice Warning: Make sure to read the following advice prior to install or to use the device and the software. If you do not fulfill all requirements stated below, this may lead to malfunctions or breakage of connected hardware, or even fatal injuries. Warning: Obey always the rules of local regulations and laws. Only qualified personnel should be allowed to perform laboratory work. Work according to good laboratory practice to obtain best results and to minimize risks.
PH01 Manual 2 Installation and Operation 2.1 Welcome to the PH01 — Perfusion Cannula with Heating Element and Sensor Cell lines and tissue slices are placed and cultivated directly in the MEA culture chamber, on the microelectrode array.
PH01 Manual 2.2 Setting Up and Connecting the PH01 Warning: Do not operate the PH01 unless it is completely filled with liquid. Otherwise, overheating may irreversibly damage the PH01. Warning: Do not start the perfusion until you have double-checked that the perfusion lines are set up properly and that the inflow and outflow rate are matching. Spilled liquid may irreversibly damage electronic instruments. If the setpoint temperature is changed, the controller will need a short time to respond.
PH01 Manual 7. Set the inflow rate to the optimum operating range (500 μl/min to 4500 μl/min). Make sure the programmed outflow rate of the aspiration system is identical (or slightly higher) than the inflow rate. For example, you can use a double-head peristaltic pump, and use one channel of the pump for the input and the other for the output. An alternative would be a push-pull pump that holds paired syringes, with one syringe for the inflow and one for the outflow. 8. Carefully double-check the setup.
PH01 Manual 2.4 Air Drafts and Ambient Temperature Ensure that the setup is shielded from air drafts. Even the smallest degree of turbulences in the air — such as when a person walks by — can affect the PH01's temperature control and thus the outlet liquid temperature as well. Ensure that the ambient temperature remains as constant as possible. Pump Types The differences between peristaltic and syringe pumps are shown here for comparison.
PH01 Manual 2.6 Flow Stream A titanium wire inserted into the cannula ensures a turbulent flow. In turbulent flow, there are no discrete layers of flowing liquid. The momentum of the fluid overcomes the viscous shear forces, and there is extensive and continual mixing across the flow stream. This ensures that the temperature of the liquid is constant across the flow stream.
