User Manual

MEA Manual

 MEA Handling 35

5.1 Hydrophilic Surface Treatment 35

5.1.1 Plasma Cleaning 35

5.1.2 Protein Coating 35

5.1.3 Preculturing 36

5.2 Sterilization 36

5.2.1 Sterilization with Ethanol and UV Light 36

5.2.2 Steam Sterilization (Autoclavation) 36

5.2.3 Dry-Heat Sterilization 36

5.2.4 Sterilization with Hot Water 36

5.3 MEA Storage 37

5.4 MEA Coating 37

5.4.1 Coating with Nitrocellulose 37

5.4.2 Coating with Polyethyleneimine (PEI) plus Laminin 38

5.4.3 Coating with Polyornithine (plus Laminin) 39

5.4.4 Coating with Poly-D-Lysine (plus Laminin) 40

5.4.5 Coating with Poly-D-Lysine (plus Fibronectin) 41

5.4.6 Coating with Fibronectin 41

5.4.7 Coating with Collagen 42

5.5 Cleaning of used MEAs 43

5.5.1 General Recommendations for Cleaning MEAs 43

5.5.2 Cleaning of pMEAs 43

5.5.3 Cleaning of EcoMEAs 43

5.5.4 Cleaning of EcoFlexMEAs 43

5.5.5 Cleaning of FlexMEAs 44

5.5.6 Removing Nitrocellulose Coating 44

5.5.7 MEA Cleaning with EDTA-Collagenase 44

5.5.8 MEA Cleaning with Terg-A-Zyme 45

6 Culture Chamber Options 47

6.1 MEA2100-CO2-C 47

6.2 Sealed MEA Culture Dish 47

6.3 MEA Culture Chamber with Lid 48

6.4 Culture Chamber for 9-Well MEAs 48

6.5 Culture Chamber for 6-Well MEAs 48