Manual

HiClamp Manual
iv
6
Data Analysis 39
6.1 Data File Organization 39
6.2 DataMining 40
6.2.1 Measuring the current amplitudes 43
6.2.2 The e-Labbook of the HiClamp 45
6.3 DataMerger 46
6.3.1 Statistic 47
6.3.2 Example of a curve fitting 47
6.3.3 Example of curve fitting using “Global Fit” 52
7 Examples 55
7.1 The voltage activation curve of Kv1.4 55
Current-Voltage activation curve for KV1.4 56
7.2 The voltage activation curve of NaV 1.5 58
8 Troubleshooting 61
8.1 Troubleshooting 61
9 Service and Maintenance 63
9.1 Service and Maintenance 63
9.1.1 Cleaning of the Basket 63
9.1.2 Replacing or Realigning 63
9.2 Preparation of Oocytes 65
9.3 Plating Oocytes 66
10 TEVC Recording Background 67
10.1 TEVC Recording Background 67
11 Appendix 69
11.1 Technical Support 69
11.2 Technical Specifications 70
11.3 About Preparation of Xenopus Oocytes 71
11.3.1 Materials 71
11.3.2 Chemicals 72
11.3.3 Oocyte Removal 72
11.3.4 Collagenase Digestion and Defolliculation 73
11.3.5 Selecting Good Oocytes 73
11.3.6 Plating Oocytes 74
11.4 Contact Information 75