Manual

iii
Table of Contents
1 Introduction 1
1.1 About this Manual 1
2 Important Information and Instructions 3
2.1 Guarantee and Liability 3
2.2 Operator's Obligations 3
2.3 Important Safety Advice 4
2.3.1 High Voltage 4
2.3.2 Requirements for the installation 4
2.3.3 Handling of the motor driven axes 5
2.3.4 Handling of the chlorided silver wires 5
2.3.5 Handling of recording electrode needles 5
3 HiClamp 7
3.1 Welcome to the HiClamp 7
3.2 Hardware 9
3.3 Setting up the HiClamp 10
3.4 Mechanical Functionality of the HiClamp 12
4 Software 17
4.1 Program Concept 17
4.2 Installing the HiClamp Software 17
4.3 Starting the HiClamp 18
5 TEVC Recording Protocol 19
5.1 Preparations for Recording 19
5.2 Electrode Alignment 20
5.3 Test Model Cell 22
5.3.1 Testing the Amplifier with the Test Model Cell 22
5.3.2 Tuning the Amplifier 23
5.4 Selecting Oocytes for TEVC Recording 26
5.5 Starting a Recording Sequence 26
5.5.1 A first and simple example 26
5.6 Icons and the associated Effects 29
5.7 Data Acquisition Icons 34
5.7.1 Measuring multiple oocytes 35
5.7.2 The concentration activation curve 36
5.7.3 Recording Voltage-Gated Channel activity 36