User guide

Experimental flowchart for two-subunit protein expression
Introduction
The diagram below schematically depicts the steps necessary to express your two-
subunit protein of interest using the Freedom
™
DG44 Kit, and it shows several
common pathways from stable transfectants to clone scale-up. Note that the times
shown for various experimental steps are approximations, and the actual times
depend on your protein of interest and the specific workflows you choose.
See Methods for Two-subunit Protein Expression, pages 20–41 for detailed
protocols.
Note: For detailed information on cloning each subunit of your protein of interest
into pOptiVEC
™
-TOPO
®
TA and pcDNA
™
3.3-TOPO
®
TA vectors, refer to the
pOptiVEC
™
-TOPO
®
TA Cloning Kit and pcDNA
™
3.3-TOPO
®
TA Cloning Kit
manuals (part nos. 25-0977 and 25-1010, respectively) that are available at
www.lifetechnologies.com/manuals.
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