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References
Andersson, S., Davis, D. L., Dahlbäck, H., Jörnvall, H., and Russell, D. W. (1989) Cloning, Structure, and
Expression of the Mitochondrial Cytochrome P-450 Sterol 26-Hydroxylase, a Bile Acid
Biosynthetic Enzyme. J. Biol. Chem. 264, 8222-8229
Boshart, M., Weber, F., Jahn, G., Dorsch-Häsler, K., Fleckenstein, B., and Schaffner, W. (1985) A Very
Strong Enhancer is Located Upstream of an Immediate Early Gene of Human Cytomegalovirus.
Cell 41, 521-530
Cole, C. N., and Stacy, T. P. (1985) Identification of Sequences in the Herpes Simplex Virus Thymidine
Kinase Gene Required for Efficient Processing and Polyadenylation. Mol. Cell. Biol. 5, 2104-2113
Gurtu, V., Yan, G., and Zhang, G. (1996) IRES bicistronic expression vectors for efficient creation of stable
mammalian cell lines. Biochem. Biophys. Res. Comm. 229, 295-298
Hennighausen, L., and Fleckenstein, B. (1986) Nuclear factor 1 interacts with five DNA elements in the
promoter region of the human cytomegalovirus major immediate early gen. Embo J 5, 1367-1371
Kaufman, R., Wasley, L., Spiliotes, A., Gossels, S., Latt, S., Larsen, G., and Kay, R. (1985) Coamplification
and coexpression of human tissue-type plasminogen activator and murine dihydrofolate
reductase in Chinese hamster ovary cells. Mol Cell Biol 5, 1750-1759
Nelson, J. A., Reynolds-Kohler, C., and Smith, B. A. (1987) Negative and Positive Regulation by a Short
Segment in the 5´-Flanking Region of the Human Cytomegalovirus Major Immediate-Early Gene.
Molec. Cell. Biol. 7, 4125-4129
Puck, T. T., Cieciura, S. J., and Robinson, A. (1958) Genetics of somatic mammalian cells. III. Long-term
cultivation of euploid cells from human and animal subjects. J Exp Med 108, 945-956
Rees, S., Coote, J., Stables, J., Goodson, S., Harris, S., and Lee, M. (1996) Bicistronic vector for the creation
of stable cell lines that predisposes all antibiotic-resistant cells to express recombinant protein.
Biotechniques 20, 102-110
Sheeley, D. M., Merrill, B. M., and Taylor, L. C. (1997) Characterization of monoclonal antibody
glycosylation: comparison of expression systems and identification of terminal alpha-linked
galactose. Anal Biochem 247, 102-110.
Southern, P. J., and Berg, P. (1982) Transformation of Mammalian Cells to Antibiotic Resistance with a
Bacterial Gene Under Control of the SV40 Early Region Promoter. J. Molec. Appl. Gen. 1, 327-339
Tanaka, H., Tapscott, S., Trask, B., and Yao, M.-C. (2002) Short inverted repeats initiate gene amplification
through the formation of a large DNA palindrome in mammalian cells. PNAS 99, 8772-8777
Urlaub, G., Kas, E., Carothers, A., and Chasin, L. (1983) Deletion of the Diploid Dihydrofolate reductase
Locus from Cultured Mammalain Cells. Cell 33, 405-412
Urlaub, G., Mitchell, P. J., Kas, E., Chasin, L. A., Funanage, V. L., Myoda, T. T., and HamLin, J. (1986)
Effect of gamma rays at the dihydrofolate reductase locus: deletions and inversions. Somat Cell
Mol Genet 12, 555-566
Werner, R. G., Noe, W., Kopp, K., and Schluter, M. (1998) Appropriate mammalian expression systems
for biopharmaceuticals. Arzneimittelforschung 48, 870-880.
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