User guide

Expressing two-subunit proteins without gene amplification
Introduction
The flowchart below depicts the major steps to express your two-subunit protein in
suspension CHO DG44 Cells (cGMP banked) with the combination of pOptiVEC
™
and pcDNA
™
3.3 constructs that gave you the highest yield.
Page
Time Line
Notes
Transfection:
1 day
page 26
page 28
Start 2–3 days
post-
transfection;
usually lasts
12–14 days
Clone
selection:
12–14 days
page 37
22