Operation Manual
61
NOMENCLATURE
Excitation Filter - EX
A filter used in fluorescence microscopy designed to pass only those wavelengths, which excite
fluorescence.
Excitation Filter D350/50x
The center wavelength is at 350nm; full bandwidth is 50nm [= ±25nm].
In some cases when the bandwidth is not specified, the letter "x" is used to define the filter as an exciter
filter and is generally used for narrow band UV excitation filters, e.g. D350x.
Dichroic Mirror - DM
The dichroic mirror is the optical component that separates the excitation light from the fluorescence.
Dichroic mirror is designed to reflect selectively the shorter wavelength exciting radiation and transmit
the longer wavelength fluorescence.
Dichroic mirror is placed in a 45° incidence angle to light, allowing the mirrors to act as a precise
discriminator of excitation and fluorescence wavelengths.
Light passing through the excitation filter is reflected 90° toward the objective and the specimen.
Finally, light emanating from the specimen is passed through and directed toward the emission filter
and observation method.
Dichroic multi-layer thin-film coatings makeup typically consists of one of the following design types:
short wave pass, long wave pass, or bandpass filter. These design types comprise the basis of color
determination and color separation and this are determined by the transmittance and/or reflectance of a
band of wavelengths.
Dichroic Beam splitter 505DCLP
The cut-on wavelength is approximately 505nm for this dichroic longpass application.
Emission Filter - EM (Barrier filter)
Emission filter is the last component in a fluorescence cube, which transmits fluorescence emission
wavelengths while blocking excitation wavelengths. Emissions filters are coloured glass or interference
filters that have transmission properties of a bandpass or longpass filters.
Most interference are mounted at a slight angle to allow for better imaging by suppressing ghost
images.
Emission filter D460/50m*
The center wavelength is at 460nm; full band is 50nm [= ±25nm].
*m –emission