Operation Manual

60
ARRANGEMENT OF FILTERS IN A FLUORESCENCE CUBE
Upright Microscope
It is often the case that specific combinations of excitation filter, emission filter and dichroic mirror
areneeded to visualize and/or quantitate the fluorescence emission from a particular fluorescent species.
In newer models of fluorescence microscopes, manufacturers have provided a means to change these
optical elements in a convenient manner by arranging a set of four or more filter cubes in a circular (or
linear) turret under the objective. With a turret arrangement, a specific filter cube can be selected in a
manner similar to that of selecting a specific objective.