Operation Manual

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Selecting fluorescence filters requires a thorough understanding of filter technology. This will enable the
user to utilize stain and illumination selection to improve the image quality of the desired fluorescence
signal.
Selection of filter combinations also requires knowledge of the excitation and emission spectra of the
stain.
Epi-Fluorescence and Phase Contrast Microscopy
In addition to Epi-Fluorescence microscopy, phase contrast microscopy can be used to search an
organelle or the location of a particular molecule in a cell instead of Epi-Fluorescence microscopy which
bleaches the specimen.
Combing Epi-Fluorescence microscopy with phase contrast microscopy makes it possible to
compensate for the limitations of each method.