Operation Manual

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4.3.1 Longpass
Longpass filters only allow wavelengths above a certain
Wavelength to pass through and will prevent light of lower
wavelength from passing through.
Longpass filters should be used when the application requires
maximum emission and spectral differentiation is not necessary.
This is generally the case for fluorophores that generate a single
emitting species in specimens with reasonably low levels of
background auto fluorescence.
Fluororophore or fluorescent probe:
Fluorochromes when conjugated to other originally active substances, such as a protein, antibody or
nucleic acid, in order to selectively stain targeted substances within the specimen
Long pass filters are also useful for observing all the stains on multi-stained specimen such as FITC and
TRITC.
4.3.2 Bandpass
Filters that pass light of a certain restricted range of wavelength.
Bandpass filters are useful for observing a certain stain on multi-stained specimen such as when two
stains FITC and TRITC are used.
Bandpass filters are designed to maximize the brightness of the desired fluorescence (signal) to
brightness of the background (the noise) ratio for applications where discrimination of signal component
is more important than overall image brightness.